Neuronal Proteomic Analysis Of The Ubiquitinated Substrates Of The Disease-Linked E3 Ligases Parkin And Ube3a
dc.contributor.author | Martínez Zárate, Aitor | |
dc.contributor.author | Ramírez Sánchez, Juan Manuel | |
dc.contributor.author | Osinalde Moraleja, Nerea | |
dc.contributor.author | Arizmendi Bastarrika, Jesús María | |
dc.contributor.author | Mayor Martínez, Ugo | |
dc.date.accessioned | 2018-06-28T08:51:58Z | |
dc.date.available | 2018-06-28T08:51:58Z | |
dc.date.issued | 2018-03-06 | |
dc.identifier.citation | Biomed Research International 2018 : (2018) // Article ID 3180413 | es_ES |
dc.identifier.issn | 2314-6133 | |
dc.identifier.issn | 2314-6141 | |
dc.identifier.uri | http://hdl.handle.net/10810/27775 | |
dc.description.abstract | Both Parkin and UBE3A are E3 ubiquitin ligases whose mutations result in severe brain dysfunction. Several of their substrates have been identified using cell culture models in combination with proteasome inhibitors, but not in more physiological settings. We recently developed the (bio)Ub strategy to isolate ubiquitinated proteins in flies and have now identified by mass spectrometry analysis the neuronal proteins differentially ubiquitinated by those ligases. This is an example of how flies can be used to provide biological material in order to reveal steady state substrates of disease causing genes. Collectively our results provide new leads to the possible physiological functions of the activity of those two disease causing E3 ligases. Particularly, in the case of Parkin the novelty of our data originates from the experimental setup, which is not overtly biased by acute mitochondrial depolarisation. In the case of UBE3A, it is the first time that a nonbiased screen for its neuronal substrates has been reported. | es_ES |
dc.description.sponsorship | The authors thank Michael Clague for insightful comments on an early version of the manuscript. Ugo Mayor, Nerea Osinalde, and Jesus M. Arizmendi are supported by the Spanish MINECO (Grant no. SAF2016-76898-P). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Hindawi | es_ES |
dc.relation | Info:eu-repo/grantAgreement/MINECO/SAF2016-76898-P | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | fusion-protein gene | es_ES |
dc.subject | fat-facets gene | es_ES |
dc.subject | angelman-syndrome | es_ES |
dc.subject | drosophila-melanogaster | es_ES |
dc.subject | mass-spectrometry | es_ES |
dc.subject | conjugating enzyme | es_ES |
dc.subject | cell-death | es_ES |
dc.subject | in-vivo | es_ES |
dc.subject | mitochondrial depolarization | es_ES |
dc.subject | neurodegenerative diseases | es_ES |
dc.title | Neuronal Proteomic Analysis Of The Ubiquitinated Substrates Of The Disease-Linked E3 Ligases Parkin And Ube3a | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | Copyright © 2018 Aitor Martinez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Attribution 4.0 International (CC BY 4.0) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.hindawi.com/journals/bmri/2018/3180413/ | es_ES |
dc.identifier.doi | 10.1155/2018/3180413 | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |
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Except where otherwise noted, this item's license is described as Copyright © 2018 Aitor Martinez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Attribution 4.0 International (CC BY 4.0)