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dc.contributor.authorSáenz del Burgo Martínez, Laura ORCID
dc.contributor.authorCiriza Astrain, Jesús
dc.contributor.authorEspona Noguera, Albert
dc.contributor.authorXavier, Illa
dc.contributor.authorCabruja Casas, Enric
dc.contributor.authorOrive Arroyo, Gorka
dc.contributor.authorHernández Martín, Rosa María ORCID
dc.contributor.authorVilla, Rosa
dc.contributor.authorPedraz Muñoz, José Luis ORCID
dc.contributor.authorÁlvarez, Mar
dc.date.accessioned2018-10-31T16:12:18Z
dc.date.available2018-10-31T16:12:18Z
dc.date.issued2018-05-31
dc.identifier.citationScientific Reports 8 : (2018) // Article ID 8512es_ES
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10810/29449
dc.description.abstractCell microencapsulation is an attractive strategy for cell-based therapies that allows the implantation of genetically engineered cells and the continuous delivery of de novo produced therapeutic products. However, the establishment of a way to retrieve the implanted encapsulated cells in case the treatment needs to be halted or when cells need to be renewed is still a big challenge. The combination of micro and macroencapsulation approaches could provide the requirements to achieve a proper immunoisolation, while maintaining the cells localized into the body. We present the development and characterization of a porous implantable macrocapsule device for the loading of microencapsulated cells. The device was fabricated in polyamide by selective laser sintering (SLS), with controlled porosity defined by the design and the sintering conditions. Two types of microencapsulated cells were tested in order to evaluate the suitability of this device; erythropoietin (EPO) producing C2C12 myoblasts and Vascular Endothelial Growth Factor (VEGF) producing BHK fibroblasts. Results showed that, even if the metabolic activity of these cells decreased over time, the levels of therapeutic protein that were produced and, importantly, released to the media were stable.es_ES
dc.description.sponsorshipThis work was done under the BIOPAN project (CIBER-BBN). Authors wish to thank the intellectual and technical assistance from the ICTS "NANBIOSIS", more specifically by the Drug Formulation Unit (U10) and the Micro-Nano Technology Unit (U8) of the CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBERBBN). Also, they thank the support to research on cell microencapsulation from the University of the Basque Country UPV/EHU (EHUA 16/06) and the Basque Country Government (Grupos Consolidados, No ref: IT907-16). The authors acknowledge the financial support from the Ministerio de Economia y Competitividad (MINECO) (Spain) through Ramon y Cajal program (RYC-2013-14479). This work has made use of the Spanish ICTS Network MICRONANOFABS partially supported by MINECO.es_ES
dc.language.isoenges_ES
dc.publisherNature Publishing Groupes_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/(RYC-2013-14479)es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectendothelial growth-factores_ES
dc.subjectmesenchymal stem-cellses_ES
dc.subjectpancreatic-isletses_ES
dc.subjecttransplantationes_ES
dc.subjectdeliveryes_ES
dc.subjectVEGFes_ES
dc.subjectdifferentiationes_ES
dc.subjectvascularizationes_ES
dc.subjectmyoblastses_ES
dc.subjecthypoxiaes_ES
dc.title3D Printed Porous Polyamide Macrocapsule Combined with Alginate Microcapsules for Safer Cell-Based Therapieses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttp://www.nature.com/articles/s41598-018-26869-5es_ES
dc.identifier.doi10.1038/s41598-018-26869-5
dc.departamentoesFarmacia y ciencias de los alimentoses_ES
dc.departamentoeuFarmazia eta elikagaien zientziakes_ES


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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Except where otherwise noted, this item's license is described as Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.