Show simple item record

dc.contributor.authorSzabó, Áron
dc.contributor.authorPapin, Christian
dc.contributor.authorCornu, David
dc.contributor.authorChélot, Elisabeth
dc.contributor.authorLipinszki, Zoltán
dc.contributor.authorUdvardy, Andor
dc.contributor.authorRedeker, Virginie
dc.contributor.authorMayor Martínez, Ugo
dc.contributor.authorRouyer, François
dc.identifier.citationCell Reports 23(8) : 2273-2282 (2018)es_ES
dc.description.abstractCircadian clocks have evolved as time-measuring molecular devices to help organisms adapt their physiology to daily changes in light and temperature. Transcriptional oscillations account for a large fraction of rhythmic protein abundance. However, cycling of various posttranslational modifications, such as ubiquitylation, also contributes to shape the rhythmic protein landscape. In this study, we used an in vivo ubiquitin labeling assay to investigate the circadian ubiquitylated proteome of Drosophila melanogaster. We find that cyclic ubiquitylation affects MEGATOR (MTOR), a chromatin-associated nucleoporin that, in turn, feeds back to regulate the core molecular oscillator. Furthermore, we show that the ubiquitin ligase subunits CULLIN-3 (CUL-3) and SUPERNUMERARY LIMBS (SLMB) cooperate for ubiquitylating the TIMELESS protein. These findings stress the importance of ubiquitylation pathways in the Drosophila circadian clock and reveal a key component of this system.es_ES
dc.description.sponsorshipWe thank A. Chatterjee and S. Andreazza for helping with the fly work, B. Grima for sharing results about other Ub purification methods, and M. Boudinot for the FaasX software. We also thank J. Jiang for the s/mbm stock, C.T. Chien for cul-3 stocks, N. Glossop for Clk-GAL4 lines, and F.R. Jackson for unpublished datasets on the translatome of tim+ cells. We are very grateful to Mads Gyrd-Hansen for the Ub chain type antibodies; C.P. Verrijzer for the MTOR antibody; and R. Paro, N. Gay, G. Beitel, A. Ferrus, J. Mueller, F. Peronnet, and R. Bauer for other antibodies. This study was supported by the following grants: DrosoClock, ClockGene, and FunGenDroso (ANR); Equipe FRM (FRM) and EUCLOCK and INsecTIME (EU 6th and 7th Framework Programs) to F.R. and GINOP-2.3.2-15-2016-00001 (Ministry for National Economy of Hungary) and OTKA-PD115404 (National Research, Development and Innovation Office) to Z.L. U.M. is a recipient of MINECO grant SAF2013-44782-P (co-financed by FEDER funds). F.R. and V.R. are supported by INSERM.es_ES
dc.publisherCell Presses_ES
dc.subjectspectrometry-based proteomicses_ES
dc.titleUbiquitylation Dynamics of the Clock Cell Proteome and TIMELESS During a Circadian Cyclees_ES
dc.rights.holderCell Reports 23, 2273–2282, May 22, 2018 ª 2018 The Author(s). 2273 This is an open access article under the CC BY-NC-ND license (
dc.rights.holderAtribución-NoComercial-SinDerivadas 3.0 España*
dc.departamentoesBioquímica y biología moleculares_ES
dc.departamentoeuBiokimika eta biologia molekularraes_ES

Files in this item


This item appears in the following Collection(s)

Show simple item record

Cell Reports 23, 2273–2282, May 22, 2018 ª 2018 The Author(s). 2273
This is an open access article under the CC BY-NC-ND license (
Except where otherwise noted, this item's license is described as Cell Reports 23, 2273–2282, May 22, 2018 ª 2018 The Author(s). 2273 This is an open access article under the CC BY-NC-ND license (