PR-LncRNA Signature Regulates Glioma Cell Activity Through Expression of SOX Factors

Torres Bayona, Sergio Andrés; Aldaz Donamaría, Paula; Auzmendi Iriarte, Jaione; Sáenz Antoñanzas, Ander; García Camino, Idoia; Arrazola Schlamilch, Mariano; Gerovska, Daniela; Undabeitia Huertas, José; Querejeta Ayerra, Arrate; Egaña Otaño, Larraitz; Villanua Bernues, Jorge Alberto; Ruiz Díaz, Irune; Sarasqueta Eizaguirre, Cristina; Urculo Bareño, Enrique; Araúzo Bravo, Marcos J.; Huarte Martínez, Maite; Samprón Lebed, Nicolás; Matheu Fernández, Ander

dc.contributor.author	Torres Bayona, Sergio Andrés
dc.contributor.author	Aldaz Donamaría, Paula
dc.contributor.author	Auzmendi Iriarte, Jaione
dc.contributor.author	Sáenz Antoñanzas, Ander
dc.contributor.author	García Camino, Idoia
dc.contributor.author	Arrazola Schlamilch, Mariano
dc.contributor.author	Gerovska, Daniela
dc.contributor.author	Undabeitia Huertas, José
dc.contributor.author	Querejeta Ayerra, Arrate
dc.contributor.author	Egaña Otaño, Larraitz
dc.contributor.author	Villanua Bernues, Jorge Alberto
dc.contributor.author	Ruiz Díaz, Irune
dc.contributor.author	Sarasqueta Eizaguirre, Cristina
dc.contributor.author	Urculo Bareño, Enrique
dc.contributor.author	Araúzo Bravo, Marcos J.
dc.contributor.author	Huarte Martínez, Maite
dc.contributor.author	Samprón Lebed, Nicolás
dc.contributor.author	Matheu Fernández, Ander
dc.date.accessioned	2018-12-12T13:12:39Z
dc.date.available	2018-12-12T13:12:39Z
dc.date.issued	2018-08-24
dc.identifier.citation	Scientific Reports 8 : (2018) // Article ID 12746	es_ES
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10810/30289
dc.description.abstract	Long non-coding RNAs (LncRNAs) have emerged as a relevant class of genome regulators involved in a broad range of biological processes and with important roles in tumor initiation and malignant progression. We have previously identified a p53-regulated tumor suppressor signature of LncRNAs (PR-LncRNAs) in colorectal cancer. Our aim was to identify the expression and function of this signature in gliomas. We found that the expression of the four PR-LncRNAs tested was high in human low-grade glioma samples and diminished with increasing grade of disease, being the lowest in glioblastoma samples. Functional assays demonstrated that PR-LncRNA silencing increased glioma cell proliferation and oncosphere formation. Mechanistically, we found an inverse correlation between PR-LncRNA expression and SOX1, SOX2 and SOX9 stem cell factors in human glioma biopsies and in glioma cells in vitro. Moreover, knock-down of SOX activity abolished the effect of PR-LncRNA silencing in glioma cell activity. In conclusion, our results demonstrate that the expression and function of PR-LncRNAs are significantly altered in gliomagenesis and that their activity is mediated by SOX factors. These results may provide important insights into the mechanisms responsible for glioblastoma pathogenesis.	es_ES
dc.description.sponsorship	PA, JA-I and AS-A were recipients of a predoctoral fellowship from the Spanish Association Against Cancer (AECC Gipuzkoa), Basque Government and Instituto Salud Carlos III. This work was supported by grants from the Carlos III Institute of Health and the European Regional Development Fund (PI13/02277, CP16/00039, DTS16/084, and PI16/01580) and Industry and Health Departments of the Basque Country.	es_ES
dc.language.iso	eng	es_ES
dc.publisher	Nature Publishing Group	es_ES
dc.rights	info:eu-repo/semantics/openAccess	es_ES
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/es/	*
dc.subject	long noncoding RNAs	es_ES
dc.subject	adjuvant temozolomide	es_ES
dc.subject	transcription factors	es_ES
dc.subject	glioblastoma	es_ES
dc.subject	STEM	es_ES
dc.subject	cancer	es_ES
dc.subject	radiotherapy	es_ES
dc.subject	pathogenesis	es_ES
dc.subject	concomitant	es_ES
dc.subject	inhibition	es_ES
dc.title	PR-LncRNA Signature Regulates Glioma Cell Activity Through Expression of SOX Factors	es_ES
dc.type	info:eu-repo/semantics/article	es_ES
dc.rights.holder	Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.	es_ES
dc.rights.holder	Atribución 3.0 España	*
dc.relation.publisherversion	https://www.nature.com/articles/s41598-018-30836-5	es_ES
dc.identifier.doi	10.1038/s41598-018-30836-5
dc.departamentoes	Cirugía, radiología y medicina física	es_ES
dc.departamentoes	Dermatología, oftalmología y otorrinolaringología	es_ES
dc.departamentoeu	Dermatologia, oftalmologia eta otorrinolaringologia	es_ES
dc.departamentoeu	Kirurgia,erradiologia eta medikuntza fisikoa	es_ES

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Except where otherwise noted, this item's license is described as Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.