Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium
dc.contributor.author | Llamas Arriba, María Goretti | |
dc.contributor.author | Pérez Ramos, Adrián | |
dc.contributor.author | Puertas González, Ana Isabel | |
dc.contributor.author | López García, Paloma | |
dc.contributor.author | Dueñas Chasco, María Teresa | |
dc.contributor.author | Prieto Orzanco, Alicia | |
dc.date.accessioned | 2019-01-09T14:05:38Z | |
dc.date.available | 2019-01-09T14:05:38Z | |
dc.date.issued | 2018-09-04 | |
dc.identifier.citation | Frontiers in Psychology 9 : (2018) // Article ID 2041 | es_ES |
dc.identifier.issn | 1664-302X | |
dc.identifier.uri | http://hdl.handle.net/10810/30697 | |
dc.description.abstract | Pediococcus ethanolidurans CUPV141 is an exopolysaccharide (EPS)-producing lactic acid bacterium, first isolated from Basque Country cider (Spain). Physicochemical analysis of the EPS synthesized by the bacterium revealed that CUPV141 produces mostly a homopolysaccharide (HoPS), characterized as a 2-substituted (1,3)-beta-D-glucan, together with a small quantity of a heteropolysaccharide (HePS) composed of glucose, galactose, glucosamine, and glycerol-3-phosphate, this being the first Pediococcus strain described to produce this kind of polymer. On the contrary, an isogenic strain CUPV141NR, generated by chemical mutagenesis of CUPV141, produced the HePS as the main extracellular polysaccharide and a barely detectable amount of 2-substituted (1,3)-beta-D-glucan. This HoPS is synthesized by the transmembrane GTF glycosyltransferase (GTF), encoded by the gtf gene, which has been previously reported to be located in the pPP2 plasmid of the Pediococcus parvulus 2.6 strain. Southern blot hybridization revealed that in CUPV141 the gtf gene is located in a plasmid designated as pPE3, whose molecular mass (34.4 kbp) is different from that of pPP2 (24.5 kbp). Analysis of the influence of the EPS on the ability of the producing bacteria to adhere to the eukaryotic Caco-2 cells revealed higher affinity for the human enterocytes of CUPV141NR compared to that of CUPV141. This result indicates that, in contrast to the 2.6 strain, the presence of the HoPS does not potentiate the binding ability of P. ethanolidurans. Moreover, it supports that the phosphate-containing bacterial HePS improved the interaction between P. ethanolidurans and the eukaryotic cells. | es_ES |
dc.description.sponsorship | This work was supported by a grant from the Department of Environment, Territorial Planification, Agriculture and Fishing and the project no. IT 866-13 from the Basque Government and by the Spanish Ministry of Science and Innovation (AGL 2015-65010-C3-1-R). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers Media | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | pediococcus ethanolidurans | es_ES |
dc.subject | beta-glucan | es_ES |
dc.subject | gtf | es_ES |
dc.subject | heteropolysaccharides | es_ES |
dc.subject | priming-glycosyltransferase | es_ES |
dc.subject | plasmid | es_ES |
dc.subject | adhesion | es_ES |
dc.subject | priming glycosyltransferase | es_ES |
dc.subject | streptococcus-thermophilus | es_ES |
dc.subject | lactobacillus-suebicus | es_ES |
dc.subject | functional-analysis | es_ES |
dc.subject | lactococcus-lactis | es_ES |
dc.subject | stress tolerance | es_ES |
dc.subject | caco-2 cells | es_ES |
dc.title | Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2018 Llamas-Arriba, Pérez-Ramos, Puertas, López, Dueñas and Prieto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fmicb.2018.02041/full | es_ES |
dc.identifier.doi | 10.3389/fmicb.2018.02041 | |
dc.departamentoes | Química aplicada | es_ES |
dc.departamentoeu | Kimika aplikatua | es_ES |
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Except where otherwise noted, this item's license is described as © 2018 Llamas-Arriba, Pérez-Ramos, Puertas, López, Dueñas and Prieto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.