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dc.contributor.authorPérez Ruiz, Irantzu
dc.contributor.authorMeijide de la Fuente, Susana
dc.contributor.authorHernández, María Luisa
dc.contributor.authorNavarro Lobato, Rosaura
dc.contributor.authorLarreategui, Zaloa
dc.contributor.authorFerrando, Marcos
dc.contributor.authorRuiz Larrea, María Begoña
dc.contributor.authorRuiz Sanz, José Ignacio
dc.date.accessioned2019-01-17T08:17:04Z
dc.date.available2019-01-17T08:17:04Z
dc.date.issued2018-11-27
dc.identifier.citationAntioxidants 7 : (2018) // Article ID 176es_ES
dc.identifier.issn2076-3921
dc.identifier.urihttp://hdl.handle.net/10810/30924
dc.description.abstractOxidative stress is associated with obstetric complications during ovarian hyperstimulation in women undergoing in vitro fertilization. The follicular fluid contains high levels of proteins, which are the main targets of free radicals. The aim of this work was to determine specific biomarkers of non-enzymatic oxidative modifications of proteins from follicular fluid in vivo, and the effect of ovarian stimulation with gonadotropins on these biomarkers. For this purpose, 27 fertile women underwent both a natural and a stimulated cycle. The biomarkers, glutamic semialdehyde (GSA), aminoadipic semialdehyde (AASA), N-epsilon-(carboxymethyl)lysine (CML), and N-epsilon-(carboxyethyl)lysine (CEL), were measured by gas-liquid chromatography coupled to mass spectrometry. Results showed that follicular fluid contained products of protein modifications by direct metal-catalyzed oxidation (GSA and AASA), glycoxidation (CML and CEL), and lipoxidation (CML). GSA was the most abundant biomarker (91.5%). The levels of CML amounted to 6% of the total lesions and were higher than AASA (1.3%) and CEL (1.2%). In the natural cycle, CEL was significantly lower (p < 0.05) than in the stimulated cycle, suggesting that natural cycles are more protected against protein glycoxidation. These findings are the basis for further research to elucidate the possible relevance of this follicular biomarker of advanced glycation end product in fertility programs.es_ES
dc.description.sponsorshipThis research was funded by the Basque Government, Department of Education, Universities and Research (project ref. IT687-13 and predoctoral grant to I.P.) and Department of Economic Development and Competitiveness, SPRI (refs. IG-20130001214 and IG-2014 0000837), University of the Basque Country UPV/EHU (ref. GIU16/62), and Fundacion Jesus Gangoiti (grant to S.M.).es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectassisted reproductiones_ES
dc.subjectfollicular fluides_ES
dc.subjectoxidative stresses_ES
dc.subjectaminoadipic semialdehydees_ES
dc.subjectglutamic semialdehydees_ES
dc.subjectgas chromatography-mass spectrometryes_ES
dc.subjectfemale infertilityes_ES
dc.subjectglycation end-productses_ES
dc.subjectIVFes_ES
dc.subjectagees_ES
dc.subjectidentificationes_ES
dc.subjectglycoxidationes_ES
dc.subjectresidueses_ES
dc.subjectsiteses_ES
dc.subjectragees_ES
dc.titleAnalysis of Protein Oxidative Modifications in Follicular Fluid from Fertile Women: Natural Versus Stimulated Cycleses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://www.mdpi.com/2076-3921/7/12/176es_ES
dc.identifier.doi10.3390/antiox7120176
dc.departamentoesFisiologíaes_ES
dc.departamentoeuFisiologiaes_ES


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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Except where otherwise noted, this item's license is described as This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).