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dc.contributor.authorEseberri Barace, Itziar ORCID
dc.contributor.authorMiranda Gómez, Jonatan ORCID
dc.contributor.authorLasa Elguezua, Arrate ORCID
dc.contributor.authorMosqueda Solís, Andrea
dc.contributor.authorGonzález Manzano, Susana
dc.contributor.authorSantos Buelga, Celestino
dc.contributor.authorPortillo Baquedano, María Puy ORCID
dc.date.accessioned2019-01-23T13:08:10Z
dc.date.available2019-01-23T13:08:10Z
dc.date.issued2019-01-11
dc.identifier.citationInternational Journal Of Molecular Sciences 20 : (2019) // Article ID 264es_ES
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10810/31162
dc.description.abstractQuercetin (Q) has rapid metabolism, which may make it worthwhile to focus on the potential activity of its metabolites. Our aim was to evaluate the triglyceride-lowering effects of Q metabolites in mature and pre-adipocytes, and to compare them to those induced by Q. 3T3-L1 mature and pre-adipocytes were treated with 0.1, 1 and 10 M of Q, tamarixetin (TAM), isorhamnetin (ISO), quercetin-3-O-glucuronide (3G), quercetin-3-O-sulfate (3S), as well as with 3S and quercetin-4-O-sulfate (4S) mixture (3S+4S). Triglyceride (TG) content in both cell types, as well as free fatty acid (FFA) and glycerol in the incubation medium of mature adipocytes were measured spectrophotometrically. Gene expression was assessed by RT-PCR. In mature adipocytes, Q decreased TG at 1 and 10 M, 3S metabolite at 1 and 10 M, and 3S+4S mixture at 10 M. 3S treatment modified the glucose uptake, and TG assembling, but not lipolysis or apoptosis. During differentiation, only 10 M of ISO reduced TG content, as did Q at physiological doses. In conclusion, 3S metabolite but not ISO, 3G, 4S and TAM metabolites can contribute to the in vivo delipidating effect of Q.es_ES
dc.description.sponsorshipThis research has been supported by Instituto de Salud Carlos III (CIBERobn), Basque Government (IT-572-13) and MINECO (AGL2015-64522-C2-2-R). Itziar Eseberri is a recipient of a doctoral fellowship from the University of the Basque Country. Andrea Mosqueda-Solís is a recipient of a doctoral fellowship from the CONACYT (Mexico).es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/AGL2015-64522-C2-2-Res_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectquercetines_ES
dc.subjectadipocyteses_ES
dc.subjectmetaboliteses_ES
dc.subjecttriglycerideses_ES
dc.titleEffects of Quercetin Metabolites on Triglyceride Metabolism of 3T3-L1 Preadipocytes and Mature Adipocyteses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/20/2/264es_ES
dc.identifier.doi10.3390/ijms20020264
dc.departamentoesFarmacia y ciencias de los alimentoses_ES
dc.departamentoeuFarmazia eta elikagaien zientziakes_ES


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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Except where otherwise noted, this item's license is described as This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).