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dc.contributor.authorSuárez Barrio, Carlota
dc.contributor.authorEtxebarria, Jaime
dc.contributor.authorHernáez Moya, Raquel
dc.contributor.authorDel Val Alonso, Marina
dc.contributor.authorRodríguez Astigarraga, Maddalen
dc.contributor.authorUrkaregi Etxepare, Arantza
dc.contributor.authorFreire Vallejo, Vanesa
dc.contributor.authorMorales González, María Celia
dc.contributor.authorDurán de la Colina, Juan Antonio
dc.contributor.authorVicario, Marta
dc.contributor.authorMolina, Irene
dc.contributor.authorHerrero Vanrell, Rocío
dc.contributor.authorAndollo Victoriano, María Noelia
dc.date.accessioned2019-05-09T08:08:45Z
dc.date.available2019-05-09T08:08:45Z
dc.date.issued2019-04-03
dc.identifier.citationInternational Journal of Molecular Sciences 20(7) : (2019) // Article ID 1655es_ES
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10810/32703
dc.description.abstractThe aim of this study is to assess if an adhesive biopolymer, sodium hyaluronate (NaHA), has synergistic effects with s-PRGF (a serum derived from plasma rich in growth factors and a blood derivative that has already shown efficacy in corneal epithelial wound healing), to reduce time of healing or posology. In vitro proliferation and migration studies, both in human corneal epithelial (HCE) cells and in rabbit primary corneal epithelial (RPCE) cultures, were carried out. In addition, we performed studies of corneal wound healing in vivo in rabbits treated with s-PRGF, NaHA, or the combination of both. We performed immunohistochemistry techniques (CK3, CK15, Ki67, SS4 integrin, ZO-1, alpha-SMA) in rabbit corneas 7 and 30 days after a surgically induced epithelial defect. In vitro results show that the combination of NaHA and s-PRGF offers the worst proliferation rates in both HCE and RPCE cells. Addition of NaHA to s-PRGF diminishes the re-epithelializing capability of s-PRGF. In vivo, all treatments, given twice a day, showed equivalent efficacy in corneal epithelial healing. We conclude that the combined use of s-PRGF and HaNA as an adhesive biopolymer does not improve the efficacy of s-PRGF alone in the wound healing of corneal epithelial defects.es_ES
dc.description.sponsorshipResearch study supported by grants from the University of the Basque Country UPV/EHU-Instituto Clinico Quirurgico de Oftalmologia ICQO (US16/23), and from the Basque Foundation for Health Research and Innovation BIOEF (BIO14/TP/002).es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectcornea regenerationes_ES
dc.subjectcorneal epithelial defectes_ES
dc.subjecthyaluronic acid (NaHA)es_ES
dc.subjectplasma rich plasma (PRP)es_ES
dc.subjectserum derived from plasma rich in growth factors (s-PRGF)es_ES
dc.subjectserum eye dropses_ES
dc.subjectwound healinges_ES
dc.titleHyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defectses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/20/7/1655es_ES
dc.identifier.doi10.3390/ijms20071655
dc.departamentoesBiología celular e histologíaes_ES
dc.departamentoesMatemática aplicadaes_ES
dc.departamentoeuMatematika aplikatuaes_ES
dc.departamentoeuZelulen biologia eta histologiaes_ES


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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Except where otherwise noted, this item's license is described as This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).