Manipulation of bicarbonate concentration in sperm capacitation media improves in vitro fertilisation output in porcine species
dc.contributor.author | Soriano Úbeda, Cristina | |
dc.contributor.author | Romero Aguirregomezcorta, Jon | |
dc.contributor.author | Matás, Carmen | |
dc.contributor.author | Visconti, Pablo E. | |
dc.contributor.author | García Vázquez, Francisco A. | |
dc.date.accessioned | 2019-05-16T09:07:43Z | |
dc.date.available | 2019-05-16T09:07:43Z | |
dc.date.issued | 2019-03-11 | |
dc.identifier.citation | Journal of Animal Science and Biotechnology 10 : (2019) // Article ID 19 | es_ES |
dc.identifier.issn | 2049-1891 | |
dc.identifier.uri | http://hdl.handle.net/10810/32826 | |
dc.description.abstract | BackgroundThe in vivo concentration of bicarbonate (HCO3-), one of the essential sperm capacitating effectors, varies greatly in the different environments sperm go through from cauda epididymis to the fertilisation site. On the contrary, porcine in vitro sperm capacitation and fertilisation media usually contains a standard concentration of 25mmol/L, and one of the main problems presented is the unacceptable high incidence of polyspermy. This work hypothesised that by modifying the HCO3- concentration of the medium, the output of in vitro sperm capacitation and fertilisation could be increased.ResultsOnce exposed to the capacitation medium, the intracellular pH (pH(i)) of spermatozoa increased immediately even at low concentrations of HCO3-, but only extracellular concentrations of and above 15mmol/L increased the substrates protein kinase A phosphorylation (pPKAs). Although with a significant delay, 15mmol/L of HCO3- stimulated sperm linear motility and increased other late events in capacitation such as tyrosine phosphorylation (Tyr-P) to levels similar to those obtained with 25mmol/L. This information allowed the establishment of a new in vitro fertilisation (IVF) system based on the optimization of HCO3- concentration to 15mmol/L, which led to a 25.3% increment of the viable zygotes (8.6% in the standard system vs. 33.9%).ConclusionsOptimising HCO3- concentrations allows for establishing an IVF method that significantly reduced porcine polyspermy and increased the production of viable zygotes. A concentration of 15mmol/L of HCO3- in the medium is sufficient to trigger the in vitro sperm capacitation and increase the fertilisation efficiency in porcine. | es_ES |
dc.description.sponsorship | This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO), the European Regional Development Fund (FEDER), Grants AGL2012-40180-C03-01-02 and AGL2015-66341-R), Fundacion Seneca (20040/GERM/16) and by a grant R01-HD-038082 (to P. E. V.) from the National Institutes of Health (NIH), USA. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Biomed Central | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/AGL2012-40180-C03-01-02 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/AGL2015-66341-R | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | adcy10 | es_ES |
dc.subject | boar | es_ES |
dc.subject | monospermy | es_ES |
dc.subject | PKA | es_ES |
dc.subject | soluble adenylyl-cyclase | es_ES |
dc.subject | in-vitro | es_ES |
dc.subject | tyrosine phosphorylation | es_ES |
dc.subject | polyspermic penetration | es_ES |
dc.subject | mammalian spermatozoa | es_ES |
dc.subject | signaling events | es_ES |
dc.subject | boar spermatozoa | es_ES |
dc.subject | motility | es_ES |
dc.subject | proteins | es_ES |
dc.subject | oocytes | es_ES |
dc.title | Manipulation of bicarbonate concentration in sperm capacitation media improves in vitro fertilisation output in porcine species | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://jasbsci.biomedcentral.com/articles/10.1186/s40104-019-0324-y#Bib1 | es_ES |
dc.identifier.doi | 10.1186/s40104-019-0324-y | |
dc.departamentoes | Fisiología | es_ES |
dc.departamentoeu | Fisiologia | es_ES |
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