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dc.contributor.authorZiesemer, Kirsten A.
dc.contributor.authorRamos Madrigal, Jazmín
dc.contributor.authorMann, Allison E.
dc.contributor.authorBrandt, Bernd W.
dc.contributor.authorSankaranarayanan, Krithivasan
dc.contributor.authorOzga, Andrew T.
dc.contributor.authorHoogland, Menno
dc.contributor.authorHofman, Courtney A.
dc.contributor.authorSalazar García, Domingo Carlos
dc.contributor.authorFrohlich, Bruno
dc.contributor.authorMilner, George R.
dc.contributor.authorStone, Anne C.
dc.contributor.authorAldenderfer, Mark
dc.contributor.authorLewis, Cecil M.
dc.contributor.authorHofman, Corinne L.
dc.contributor.authorWarinner, Christina
dc.contributor.authorSchroeder, Hannes
dc.date.accessioned2019-05-21T08:02:32Z
dc.date.available2019-05-21T08:02:32Z
dc.date.issued2019-03
dc.identifier.citationAmerican Journal Of Physical Anthropology 168(3) : 496-509 (2019)es_ES
dc.identifier.issn0002-9483
dc.identifier.issn1096-8644
dc.identifier.urihttp://hdl.handle.net/10810/32884
dc.description.abstractObjectives Dental calculus is among the richest known sources of ancient DNA in the archaeological record. Although most DNA within calculus is microbial, it has been shown to contain sufficient human DNA for the targeted retrieval of whole mitochondrial genomes. Here, we explore whether calculus is also a viable substrate for whole human genome recovery using targeted enrichment techniques. Materials and methods Total DNA extracted from 24 paired archaeological human dentin and calculus samples was subjected to whole human genome enrichment using in-solution hybridization capture and high-throughput sequencing. Results Total DNA from calculus exceeded that of dentin in all cases, and although the proportion of human DNA was generally lower in calculus, the absolute human DNA content of calculus and dentin was not significantly different. Whole genome enrichment resulted in up to four-fold enrichment of the human endogenous DNA content for both dentin and dental calculus libraries, albeit with some loss in complexity. Recovering more on-target reads for the same sequencing effort generally improved the quality of downstream analyses, such as sex and ancestry estimation. For nonhuman DNA, comparison of phylum-level microbial community structure revealed few differences between precapture and postcapture libraries, indicating that off-target sequences in human genome-enriched calculus libraries may still be useful for oral microbiome reconstruction. Discussion While ancient human dental calculus does contain endogenous human DNA sequences, their relative proportion is low when compared with other skeletal tissues. Whole genome enrichment can help increase the proportion of recovered human reads, but in this instance enrichment efficiency was relatively low when compared with other forms of capture. We conclude that further optimization is necessary before the method can be routinely applied to archaeological samples.es_ES
dc.description.sponsorshipH2020 European Research Council, Grant/Award Number: 319209; Horizon 2020 Framework Programme, Grant/Award Number: 649307DK-TAF 5662; National Science Foundation, Grant/Award Number: BCS 1516633, BCS-1528698es_ES
dc.language.isoenges_ES
dc.publisherWileyes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectancient DNAes_ES
dc.subjectgenomicses_ES
dc.subjecthybridization capturees_ES
dc.subjecttarget enrichmentes_ES
dc.subjectRNA gene databasees_ES
dc.subjectadmixture proportionses_ES
dc.subjectread alignmentes_ES
dc.subjectDNAes_ES
dc.subjectsequencees_ES
dc.subjectenrichmentes_ES
dc.subjectcavees_ES
dc.subjectidentificationes_ES
dc.subjectancestryes_ES
dc.subjectpatternses_ES
dc.titleThe Efficacy of Whole Human Genome Capture on Ancient Dental Calculus and Dentines_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderAttribution 4.0 International (CC BY 4.0). You are free to: Share — copy and redistribute the material in any medium or format. Adapt — remix, transform, and build upon the material for any purpose, even commercially.es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/full/10.1002/ajpa.23763es_ES
dc.identifier.doi10.1002/ajpa.23763
dc.departamentoesGeografía, prehistoria y arqueologíaes_ES
dc.departamentoeuGeografia,historiaurrea eta arkeologiaes_ES


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Attribution 4.0 International (CC BY 4.0). You are free to:
Share — copy and redistribute the material in any medium or format.
Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0). You are free to: Share — copy and redistribute the material in any medium or format. Adapt — remix, transform, and build upon the material for any purpose, even commercially.