dc.contributor.author | Espona Noguera, Albert | |
dc.contributor.author | Etxebarria Elezgarai, Jaione | |
dc.contributor.author | Sáenz del Burgo Martínez, Laura | |
dc.contributor.author | Cañibano Hernández, Alberto | |
dc.contributor.author | Gurruchaga Iribar, Haritz | |
dc.contributor.author | Blanco, Francisco Javier | |
dc.contributor.author | Orive Arroyo, Gorka | |
dc.contributor.author | Hernández Martín, Rosa María | |
dc.contributor.author | Benito López, Fernando | |
dc.contributor.author | Ciriza Astrain, Jesús | |
dc.contributor.author | Basabe Desmonts, Lourdes | |
dc.contributor.author | Pedraz Muñoz, José Luis | |
dc.date.accessioned | 2019-11-19T19:16:44Z | |
dc.date.available | 2019-11-19T19:16:44Z | |
dc.date.issued | 2019-02-10 | |
dc.identifier.citation | International Journal of Pharmaceutics 560 : 65-77 (2019) | es_ES |
dc.identifier.issn | 0378-5173 | |
dc.identifier.uri | http://hdl.handle.net/10810/36387 | |
dc.description.abstract | Microencapsulation of pancreatic islets for the treatment of Type I Diabetes Mellitus (T1DM) generates a high quantity of empty microcapsules, resulting in high therapeutic graft volumes that can enhance the host’s immune response. We report a 3D printed microfluidic magnetic sorting device for microcapsules purification with the objective to reduce the number of empty microcapsules prior transplantation. In this study, INS1E pseudoislets were microencapsulated within alginate (A) and alginate-poly-L-lysine-alginate (APA) microcapsules and purified through the microfluidic device. APA microcapsules demonstrated higher mechanical integrity and stability than A microcapsules, showing better pseudoislets viability and biological function. Importantly, we obtained a reduction of the graft volume of 77.5% for A microcapsules and 78.6% for APA microcapsules. After subcutaneous implantation of induced diabetic Wistar rats with magnetically purified APA microencapsulated pseudoislets, blood glucose levels were restored into normoglycemia (< 200 mg/dL) for almost 17 weeks. In conclusion, our described microfluidic magnetic sorting device represents a great alternative approach for the graft volume reduction of microencapsulated pseudoislets and its application in T1DM disease. | es_ES |
dc.description.sponsorship | University of the Basque Country UPV/EHU (Spain) (EHUa16/06 to L.SB, and ESPPOC
16/65), the Basque Country Government (Spain) (Grupos Consolidados with Grant N° IT907-16 to JL. P, Elkartek with Grant N°KK-2017/ 0000088 and RIS3 with Grant N°307616FKA4) and the Spanish
Government (Spain) (RYC-2012-10796). Authors also wish to thank the intellectual and technical assistance from the ICTS “NANBIOSIS”, more specifically by the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) at the University of Basque Country UPV/EHU, Prof. Maechler from the University of Geneva Medical Center for providing the INS1E cell line, Adhesive Research for providing PSA sheets and Prof. Martínez de Pancorbo for her laboratory facilities at University of the Basque Country UPV/EHU. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/RYC-2012-10796 | es_ES |
dc.rights | info:eu-repo/semantics/embargoedAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | microcapsule sorting | es_ES |
dc.subject | alginate | es_ES |
dc.subject | superparamagnetic iron oxide nanoparticles | es_ES |
dc.subject | microfluidics | es_ES |
dc.subject | type I diabetes mellitus | es_ES |
dc.title | Type 1 Diabetes Mellitus reversal via implantation of magnetically purified microencapsulated pseudoislets | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | (c) 2019 Elsevier | es_ES |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0378517319300997 | es_ES |
dc.identifier.doi | 10.1016/j.ijpharm.2019.01.058 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | es_ES |
dc.departamentoes | Química analítica | es_ES |
dc.departamentoes | Zoología y biología celular animal | es_ES |
dc.departamentoeu | Kimika analitikoa | es_ES |
dc.departamentoeu | Zoologia eta animalia zelulen biologia | es_ES |