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dc.contributor.authorEspona Noguera, Albert
dc.contributor.authorEtxebarria Elezgarai, Jaione
dc.contributor.authorSáenz del Burgo Martínez, Laura
dc.contributor.authorCañibano Hernández, Alberto
dc.contributor.authorGurruchaga Iribar, Haritz
dc.contributor.authorBlanco, Francisco Javier
dc.contributor.authorOrive Arroyo, Gorka
dc.contributor.authorHernández Martín, Rosa María
dc.contributor.authorBenito López, Fernando
dc.contributor.authorCiriza Astrain, Jesús
dc.contributor.authorBasabe Desmonts, Lourdes
dc.contributor.authorPedraz Muñoz, José Luis
dc.identifier.citationInternational Journal of Pharmaceutics 560 : 65-77 (2019)es_ES
dc.description.abstractMicroencapsulation of pancreatic islets for the treatment of Type I Diabetes Mellitus (T1DM) generates a high quantity of empty microcapsules, resulting in high therapeutic graft volumes that can enhance the host’s immune response. We report a 3D printed microfluidic magnetic sorting device for microcapsules purification with the objective to reduce the number of empty microcapsules prior transplantation. In this study, INS1E pseudoislets were microencapsulated within alginate (A) and alginate-poly-L-lysine-alginate (APA) microcapsules and purified through the microfluidic device. APA microcapsules demonstrated higher mechanical integrity and stability than A microcapsules, showing better pseudoislets viability and biological function. Importantly, we obtained a reduction of the graft volume of 77.5% for A microcapsules and 78.6% for APA microcapsules. After subcutaneous implantation of induced diabetic Wistar rats with magnetically purified APA microencapsulated pseudoislets, blood glucose levels were restored into normoglycemia (< 200 mg/dL) for almost 17 weeks. In conclusion, our described microfluidic magnetic sorting device represents a great alternative approach for the graft volume reduction of microencapsulated pseudoislets and its application in T1DM disease.es_ES
dc.description.sponsorshipUniversity of the Basque Country UPV/EHU (Spain) (EHUa16/06 to L.SB, and ESPPOC 16/65), the Basque Country Government (Spain) (Grupos Consolidados with Grant N° IT907-16 to JL. P, Elkartek with Grant N°KK-2017/ 0000088 and RIS3 with Grant N°307616FKA4) and the Spanish Government (Spain) (RYC-2012-10796). Authors also wish to thank the intellectual and technical assistance from the ICTS “NANBIOSIS”, more specifically by the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) at the University of Basque Country UPV/EHU, Prof. Maechler from the University of Geneva Medical Center for providing the INS1E cell line, Adhesive Research for providing PSA sheets and Prof. Martínez de Pancorbo for her laboratory facilities at University of the Basque Country UPV/EHU.es_ES
dc.subjectmicrocapsule sortinges_ES
dc.subjectsuperparamagnetic iron oxide nanoparticleses_ES
dc.subjecttype I diabetes mellituses_ES
dc.titleType 1 Diabetes Mellitus reversal via implantation of magnetically purified microencapsulated pseudoisletses_ES
dc.rights.holder(c) 2019 Elsevieres_ES
dc.departamentoesFarmacia y ciencias de los alimentoses_ES
dc.departamentoesQuímica analíticaes_ES
dc.departamentoesZoología y biología celular animales_ES
dc.departamentoeuKimika analitikoaes_ES
dc.departamentoeuZoologia eta animalia zelulen biologiaes_ES

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(c) 2019 Elsevier
Except where otherwise noted, this item's license is described as (c) 2019 Elsevier