Reprogramming of gene expression in Escherichia coli cultured on pyruvate versus glucose
dc.contributor.author | Kaberdina, Anna Chao | |
dc.contributor.author | Ruiz Larrabeiti, Olatz | |
dc.contributor.author | Lin Chao, Sue | |
dc.contributor.author | Kaberdin, Vladimir | |
dc.date.accessioned | 2019-12-03T08:38:14Z | |
dc.date.available | 2019-12-03T08:38:14Z | |
dc.date.issued | 2019-07-30 | |
dc.identifier.citation | Molecular Genetics and Genomics 294(5) : 1359-1371 (2019) | es_ES |
dc.identifier.issn | 1617-4615 | |
dc.identifier.issn | 1617-4623 | |
dc.identifier.uri | http://hdl.handle.net/10810/36663 | |
dc.description.abstract | Previous studies revealed important roles of small RNAs (sRNAs) in regulation of bacterial metabolism, stress responses and virulence. However, only a minor fraction of sRNAs is well characterized with respect to the spectra of their targets, conditional expression profiles and actual mechanisms they use to regulate gene expression to control particular biological pathways. To learn more about the specific contribution of sRNAs to the global regulatory network controlling the Escherichia coli central carbon metabolism (CCM), we employed microarray analysis and compared transcriptome profiles of E. coli cells grown on two alternative minimal media supplemented with either pyruvate or glucose, respectively. Microarray analysis revealed that utilization of these alternative carbon sources led to profound differences in gene expression affecting all major gene clusters associated with CCM as well as expression of several known (CyaR, RyhB, GcvB and RyeA) and putative (C0652) sRNAs. To assess the impact of transcriptional reprogramming of gene expression on E. coli protein abundance, we also employed two-dimensional protein gel electrophoresis. Our experimental data made it possible to determine the major pathways for pyruvate assimilation when it is used as a sole carbon source and reveal the impact of other key processes (i.e., energy production, molecular transport and cell resistance to stress) associated with the CCM in E. coli. Moreover, some of these processes were apparently controlled by GcvB, RyhB and CyaR at the post-transcriptional level, thus indicating the complexity and interconnection of the regulatory networks that control CCM in bacteria. | es_ES |
dc.description.sponsorship | This work was in part supported by the Spanish Ministry of Economy and Competitiveness grants BFU2011-25455 and CGL2015-70929-R, Ikerbasque (Basque Foundation for Science), grants AS 034006 and MOST 104-2311-B-001-011-MY3 (to S. L.-C.), a predoctoral grant from the University of the Basque Country (O.R-L) and Postdoctoral Fellows Program 23-13 of Academia Sinica (A.C.K.) | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/BFU2011-25455 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/CGL2015-70929-R | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | glycolysis | es_ES |
dc.subject | gluconeogenesis | es_ES |
dc.subject | small rnas | es_ES |
dc.subject | post-transcriptional control | es_ES |
dc.subject | pyruvate | es_ES |
dc.subject | ryhb | es_ES |
dc.subject | gcvb | es_ES |
dc.subject | cyar | es_ES |
dc.subject | ryea | es_ES |
dc.subject | carbon catabolite repression | es_ES |
dc.subject | small regulatory rna | es_ES |
dc.subject | messenger-rna | es_ES |
dc.subject | metabolism | es_ES |
dc.subject | bacteria | es_ES |
dc.subject | system | es_ES |
dc.subject | translation | es_ES |
dc.subject | mechanism | es_ES |
dc.subject | activator | es_ES |
dc.subject | proteins | es_ES |
dc.title | Reprogramming of gene expression in Escherichia coli cultured on pyruvate versus glucose | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © The Author(s) 2019 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://link.springer.com/article/10.1007%2Fs00438-019-01597-1 | es_ES |
dc.identifier.doi | 10.1007/s00438-019-01597-1 | |
dc.departamentoes | Inmunología, microbiología y parasitología | es_ES |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia | es_ES |
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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.