Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids
dc.contributor.author | González Ramírez, Emilio José | |
dc.contributor.author | Goñi Urcelay, Félix María | |
dc.contributor.author | Alonso Izquierdo, Alicia | |
dc.date.accessioned | 2020-01-13T09:27:32Z | |
dc.date.available | 2020-01-13T09:27:32Z | |
dc.date.issued | 2019-09-16 | |
dc.identifier.citation | Scientific Reports 9 : (2019) // Article ID 13326 | es_ES |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10810/37731 | |
dc.description.abstract | The properties of bilayers composed of pure brain cerebroside (bCrb) or of binary mixtures of bCrb with brain ceramide, cholesterol, egg phosphatidylcholine or brain sphingomyelin have been studied using a combination of physical techniques. Pure bCrb exhibits a rather narrow gel-fluid transition centred at approximate to 65 degrees C with a half-width at half-height T-1/2 approximate to 3 degrees C. bCrb mixes well with both fluid and gel phospholipids and ceramide, and it rigidifies bilayers of egg phosphatidylcholine or brain sphingomyelin when the latter are in the fluid state. Cholesterol markedly widens the bCrb gel-fluid transition, while decreasing the associated transition enthalpy, in the manner of cholesterol mixtures with saturated phosphatidylcholines, or sphingomyelins. Laurdan and DPH fluorescence indicate the formation of fluid ordered phases in the bCrb:cholesterol mixtures. Macroscopic phase separation of more and less fluid domains is observed in giant unilamellar vesicles consisting of bCrb:egg phosphatidylcholine or bCrb:sphingomyelin. Crb capacity to induce bilayer permeabilization or transbilayer (flip-flop) lipid motion is much lower than those of ceramides. The mixtures explored here contained mostly bCrb concentrations >50 mol%, mimicking the situation of cell membranes in Gaucher's disease, or of the Crb-enriched microdomains proposed to exist in healthy cell plasma membranes. | es_ES |
dc.description.sponsorship | This work was supported in part by the Spanish Ministry of Economy (Grant No. FEDER MINECO PGC2018099857-B-I00) and the Basque Government (Grants No. IT1264-19 and IT1270-19). EJGR was a recipient of a predoctoral grant from the University of the Basque Country. The authors thank Dr. J. Sot for his continuous help and advice. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/FEDER MINECO PGC2018099857-B-I00 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | sphingomyelinase activity | es_ES |
dc.subject | biophysical properties | es_ES |
dc.subject | physical-properties | es_ES |
dc.subject | phase-behavior | es_ES |
dc.subject | lipid rafts | es_ES |
dc.subject | mixtures | es_ES |
dc.subject | glycosphingolipids | es_ES |
dc.subject | glucosylceramide | es_ES |
dc.subject | membranes | es_ES |
dc.subject | bilayers | es_ES |
dc.title | Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. (CC BY 4.0) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-019-50020-7 | es_ES |
dc.identifier.doi | 10.1038/s41598-019-50020-7 | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |
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