Regulation of boar sperm functionality by the nitric oxidesynthase/nitric oxide system
dc.contributor.author | Staicu, Florentin-Daniel | |
dc.contributor.author | Lopez Úbeda, Rebeca | |
dc.contributor.author | Romero Aguirregomezcorta, Jon ![]() | |
dc.contributor.author | Martínez Soto, Juan Carlos | |
dc.contributor.author | Matás Parra, Carmen | |
dc.date.accessioned | 2020-01-15T13:36:57Z | |
dc.date.available | 2020-01-15T13:36:57Z | |
dc.date.issued | 2019-08 | |
dc.identifier.citation | Journal of Assisted Reproduction and Genetics 36(8) : 1721-1736 (2019) | es_ES |
dc.identifier.issn | 1058-0468 | |
dc.identifier.issn | 1573-7330 | |
dc.identifier.uri | http://hdl.handle.net/10810/38474 | |
dc.description.abstract | Purpose Nitric oxide (NO) is a free radical synthesized mainly by nitric oxide synthases (NOSs). NO regulates many aspects in sperm physiology in different species. However, in vitro studies investigating NOS distribution, and how NO influences sperm capacitation and fertilization (IVF) in porcine, have been lacking. Therefore, our study aimed to clarify these aspects. Methods Two main experiments were conducted: (i) boar spermatozoa were capacitated in the presence/absence of S-nitrosoglutathione (GSNO), a NO donor, and two NOS inhibitors, N-G-nitro-L-arginine methyl ester hydrochloride (L-NAME) and aminoguanidine hemisulfate salt (AG), and (ii) IVF was performed in the presence or not of these supplements, but neither the oocytes nor the sperm were previously incubated in the supplemented media. Results Our results suggest that NOS distribution could be connected to pathways which lead to capacitation. Treatments showed significant differences after 30 min of incubation, compared to time zero in almost all motility parameters (P < 0.05). When NOSs were inhibited, three protein kinase A (PKA) substrates (similar to 75, similar to 55, and similar to 50 kDa) showed lower phosphorylation levels between treatments (P < 0.05). No differences were observed in total tyrosine phosphorylation levels evaluated by Western blotting nor in situ. The percentage of acrosome-reacted sperm and phosphatidylserine translocation was significantly lower with L-NAME. Both inhibitors reduced sperm intracellular calcium concentration and IVF parameters, but L-NAME impaired sperm ability to penetrate denuded oocytes. Conclusions These findings point out to the importance of both sperm and cumulus-oocyte-derived NO in the IVF outcome in porcine. | es_ES |
dc.description.sponsorship | This study was supported by H2020 MSC-ITN-EJD 675526 REP-BIOTECH, the Spanish Ministry of Economy and Competitiveness (MINECO), and the European Regional Development Fund (FEDER), Grant AGL2015-66341-R, and by a grant ESPDOC17/33 (to Jon Romero-Aguirregomezcorta) from the University of the Basque Country (UPV/EHU, Spain). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/AGL2015-66341-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/MSC-ITN-EJD 675526 REP-BIOTECH | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | nitric oxide | es_ES |
dc.subject | nitric oxide synthase | es_ES |
dc.subject | spermatozoa | es_ES |
dc.subject | capacitation | es_ES |
dc.subject | in vitro fertilization | es_ES |
dc.subject | protein-tyrosine phosphorylation | es_ES |
dc.subject | acrosome reaction | es_ES |
dc.subject | human spermatozoa | es_ES |
dc.subject | plasma-membrane | es_ES |
dc.subject | l-arginine | es_ES |
dc.subject | promotes capacitation | es_ES |
dc.subject | s-nitrosylation | es_ES |
dc.subject | inhibition | es_ES |
dc.subject | calcium | es_ES |
dc.subject | mouse | es_ES |
dc.title | Regulation of boar sperm functionality by the nitric oxidesynthase/nitric oxide system | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This article is distributed under the terms of the CreativeCommons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use,distribution, and reproductionin any medium, provided you giveappropriate credit to the original author(s) and the source, provide a linkto the Creative Commons license, and indicate if changes were made. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://link.springer.com/article/10.1007%2Fs10815-019-01526-6 | es_ES |
dc.identifier.doi | 10.1007/s10815-019-01526-6 | |
dc.contributor.funder | European Commission | |
dc.departamentoes | Fisiología | es_ES |
dc.departamentoeu | Fisiologia | es_ES |
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