Combined Analysis of Methylation and Gene Expression Profiles in Separate Compartments of Small Bowel Mucosa Identified Celiac Disease Patients' Signatures
dc.contributor.author | Cielo, D. | |
dc.contributor.author | Galatola, M. | |
dc.contributor.author | Fernández Jiménez, Nora ![]() | |
dc.contributor.author | De Leo, L. | |
dc.contributor.author | García Etxebarria, Koldo | |
dc.contributor.author | Loganes, C. | |
dc.contributor.author | Tommasini, A. | |
dc.contributor.author | Not, T. | |
dc.contributor.author | Auricchio, Renata | |
dc.contributor.author | Greco, Luigi | |
dc.contributor.author | Bilbao Catalá, José Ramón ![]() | |
dc.date.accessioned | 2020-02-24T09:24:16Z | |
dc.date.available | 2020-02-24T09:24:16Z | |
dc.date.issued | 2019-07-10 | |
dc.identifier.citation | Scientific Reports 9 : (2019) // Article ID 10020 | es_ES |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10810/41404 | |
dc.description.abstract | By GWAS studies on celiac disease, gene expression was studied at the level of the whole intestinal mucosa, composed by two different compartments: epithelium and lamina propria. Our aim is to analyse the gene-expression and DNA methylation of candidate genes in each of these compartments. Epithelium was separated from lamina propria in biopsies of CeD patients and CTRs using magnetic beads. Gene-expression was analysed by RT-PC; methylation analysis required bisulfite conversion and NGS. Reverse modulation of gene-expression and methylation in the same cellular compartment was observed for the IL21 and SH2B3 genes in CeD patients relative to CTRs. Bioinformatics analysis highlighted the regulatory elements in the genomic region of SH2B3 that altered methylation levels. The cREL and TNFAIP3 genes showed methylation patterns that were significantly different between CeD patients and CTRs. In CeD, the genes linked to inflammatory processes are up-regulated, whereas the genes involved in the cell adhesion/ integrity of the intestinal barrier are down-regulated. These findings suggest a correlation between gene-expression and methylation profile for the IL21 and SH2B3 genes. We identified a "gene-expression phenotype" of CeD and showed that the abnormal response to dietary antigens in CeD might be related not to abnormalities of gene structure but to the regulation of molecular pathways. | es_ES |
dc.description.sponsorship | This work was funded by the FC-Grant2013. Programma di mobilita nell'ambito delle reti di eccellenza POR Campania FSE 2007-2013 Asse V. The authors thank the European Laboratory for Food-induced Disease (ELFID). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | adapter protein | es_ES |
dc.subject | endothelial-cells | es_ES |
dc.subject | self-renewal | es_ES |
dc.subject | lnk | es_ES |
dc.subject | immune | es_ES |
dc.subject | inhibition | es_ES |
dc.subject | diagnosis | es_ES |
dc.subject | variants | es_ES |
dc.subject | cloning | es_ES |
dc.subject | signal | es_ES |
dc.title | Combined Analysis of Methylation and Gene Expression Profiles in Separate Compartments of Small Bowel Mucosa Identified Celiac Disease Patients' Signatures | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-019-46468-2#rightslink | es_ES |
dc.identifier.doi | 10.1038/s41598-019-46468-2 | |
dc.departamentoes | Genética, antropología física y fisiología animal | es_ES |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia | es_ES |
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Except where otherwise noted, this item's license is described as Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.