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dc.contributor.authorPinto, Francisco M.
dc.contributor.authorRavina, Cristina G.
dc.contributor.authorSubirán Ciudad, Nerea
dc.contributor.authorCejudo Román, Antonio
dc.contributor.authorFernández Sánchez, Manuel
dc.contributor.authorIrazusta Astiazaran, Jon ORCID
dc.contributor.authorGarrido, Nicolás
dc.contributor.authorCandenas, Luz
dc.date.accessioned2011-06-15T09:06:58Z
dc.date.available2011-06-15T09:06:58Z
dc.date.issued2010
dc.identifier.citationReproductive Biology and Endocrinology 8(104) : (2010)es
dc.identifier.issn1477-7827
dc.identifier.urihttp://hdl.handle.net/10810/4217
dc.description.abstractBackground We examined the presence and function of tachykinins and the tachykinin-degrading enzymes neprilysin (NEP) and neprilysin-2 (NEP2) in human spermatozoa. Methods Freshly ejaculated semen was collected from forty-eight normozoospermic human donors. We analyzed the expression of substance P, neurokinin A, neurokinin B, hemokinin-1, NEP and NEP2 in sperm cells by reverse-transcriptase polymerase chain reaction (RT-PCR), western blot and immunocytochemistry assays and evaluated the effects of the neprilysin and neprilysin-2 inhibitor phosphoramidon on sperm motility in the absence and presence of tachykinin receptor-selective antagonists. Sperm motility was measured using WHO procedures or computer-assisted sperm analysis (CASA). Results The mRNAs of the genes that encode substance P/neurokinin A (TAC1), neurokinin B (TAC3), hemokinin-1 (TAC4), neprilysin (MME) and neprilysin-2 (MMEL1) were expressed in human sperm. Immunocytochemistry studies revealed that tachykinin and neprilysin proteins were present in spermatozoa and show specific and differential distributions. Phosphoramidon increased sperm progressive motility and its effects were reduced in the presence of the tachykinin receptor antagonists SR140333 (NK1 receptor-selective) and SR48968 (NK2 receptor-selective) but unmodified in the presence of SR142801 (NK3 receptor-selective). Conclusion These data show that tachykinins are present in human spermatozoa and participate in the regulation of sperm motility. Tachykinin activity is regulated, at least in part, by neprilysins.es
dc.description.sponsorshipMinisterio de Educación y Ciencia (CTQ2007-61024/BQU) y Junta de Andalucía (P08-CVI-04185)es
dc.language.isoenges
dc.publisherBioMed Centrales
dc.relationinfo:eu-repo/grantAgreement/MICINN/CTQ2007-61024-BQU
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectangiotensin converting enzymees
dc.subjectmolecular cloninges
dc.subjectneurokinin Bes
dc.subjectNeutral endopeptidasees
dc.subjectzinc metallopeptidasees
dc.subjectspermatozoal RNAes
dc.subjectgene expresiones
dc.subjectimmune systemes
dc.subjectmouse testises
dc.subjecthuman semenes
dc.titleAutocrine regulation of human sperm motility by tachykininses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2010 Pinto et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.es
dc.relation.publisherversionhttp://www.rbej.com/content/8/1/104es
dc.identifier.doi10.1186/1477-7827-8-104
dc.departamentoesFisiologíaes_ES
dc.departamentoeuFisiologiaes_ES
dc.subject.categoriaDEVELOPMENTAL BIOLOGY
dc.subject.categoriaREPRODUCTIVE MEDICINE
dc.subject.categoriaENDOCRINOLOGY AND METABOLISM


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