Protein tyrosine phosphatase modulates cell growth and associates with poor outcome in human neuroblastoma
dc.contributor.author | Nunes Xavier, Caroline E. | |
dc.contributor.author | Aurtenetxe, Olaia | |
dc.contributor.author | Zaldumbide Dueñas, Laura | |
dc.contributor.author | López Almaraz, Ricardo | |
dc.contributor.author | Erramuzpe Aliaga, Asier | |
dc.contributor.author | Cortés Díaz, Jesús María | |
dc.contributor.author | López Fernández de Villaverde, José Ignacio | |
dc.contributor.author | Pulido Murillo, Rafael | |
dc.date.accessioned | 2020-03-25T12:51:14Z | |
dc.date.available | 2020-03-25T12:51:14Z | |
dc.date.issued | 2019-12-14 | |
dc.identifier.citation | Diagnostic Pathology 14(1) : (2019) // Article ID 134 | es_ES |
dc.identifier.issn | 1746-1596 | |
dc.identifier.uri | http://hdl.handle.net/10810/42326 | |
dc.description.abstract | Background Protein tyrosine phosphatases (PTPs) regulate neuronal differentiation and survival, but their expression patterns and functions in human neuroblastoma (NB) are scarcely known. Here, we have investigated the function and expression of the non-receptor PTPN1 on human NB cell lines and human NB tumor samples. Material/methods NB tumor samples from 44 patients were analysed by immunohistochemistry using specific antibodies against PTPN1, PTPRH, PTPRZ1, and PTEN. PTPN1 knock-down, cell proliferation and tyrosine phosphorylation analyses, and RT-qPCR mRNA expression was assessed on SH-SY5Y, SMS-KCNR, and IMR-32 human NB cell lines. Results Knock-down of PTPN1 in SH-SY5Y NB cells resulted in increased tyrosine phosphorylation and cell proliferation. Retinoic acid-mediated differentiation of NB cell lines did not affect PTPN1 mRNA expression, as compared with other PTPs. Importantly, PTPN1 displayed high expression on NB tumors in association with metastasis and poor prognosis. Conclusions Our results identify PTPN1 as a candidate regulator of NB cell growth and a potential NB prognostic biomarker. | es_ES |
dc.description.sponsorship | This work was partially supported by grants: BIO13/CI/001/BC from BIOEF (EITB maratoia), Basque Country, Spain; SAF2013-48812-R and SAF2016-79847-R from Ministerio de Educacion y Ciencia (to RP) and Ministerio de Economia y Competitividad (Spain and Fondo Europeo de Desarrollo Regional) (to RP and JIL); and 239813 from The Research Council of Norway (to CENX). AE was supported by a predoctoral grant from the Basque Government (Programa de Formacion de Personal Investigador no doctor, Departamento de Educacion, Politica Linguistica y Cultura del Gobierno Vasco). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | BMC | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2013-48812-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2016-79847-R | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | neuroblastoma | es_ES |
dc.subject | neuroblastoma differentiation | es_ES |
dc.subject | metastatic neuroblastoma | es_ES |
dc.subject | protein tyrosine phosphatases | es_ES |
dc.subject | ptpn1 retinoic acid | es_ES |
dc.subject | neurotrophic factor | es_ES |
dc.subject | ptp1b | es_ES |
dc.subject | expression | es_ES |
dc.subject | alk | es_ES |
dc.subject | differentiation | es_ES |
dc.subject | receptor | es_ES |
dc.subject | inhibition | es_ES |
dc.subject | cancer | es_ES |
dc.subject | survival | es_ES |
dc.title | Protein tyrosine phosphatase modulates cell growth and associates with poor outcome in human neuroblastoma | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://diagnosticpathology.biomedcentral.com/articles/10.1186/s13000-019-0919-9 | es_ES |
dc.identifier.doi | 10.1186/s13000-019-0919-9 | |
dc.departamentoes | Especialidades médico-quirúrgicas | es_ES |
dc.departamentoeu | Medikuntza eta kirurgia espezialitateak | es_ES |
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Except where otherwise noted, this item's license is described as The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.