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dc.contributor.authorDiaz de Cerio Arruabarrena, Oihane ORCID
dc.contributor.authorReina, Lander
dc.contributor.authorSquatrito, Valeria
dc.contributor.authorEtxebarria Loizate, Nestor
dc.contributor.authorGonzález Gaya, Belén
dc.contributor.authorCancio Uriarte, Ibon
dc.date.accessioned2020-04-02T17:34:31Z
dc.date.available2020-04-02T17:34:31Z
dc.date.issued2020-02-28
dc.identifier.citationBiomolecules 10(3) : (2020) // Article ID 373es_ES
dc.identifier.issn2218-273X
dc.identifier.urihttp://hdl.handle.net/10810/42590
dc.description.abstractReactive oxygen species present a challenge for marine organisms releasing gametes into the water. Thiol-containing molecules protect cells against oxidative stress, and ovothiol (OSH), an antioxidant-reducing mercaptohistidine, has been described as especially relevant in the oocytes of marine invertebrates. Ovothiol synthase (ovoA), in charge of the first step in OSH synthesis, was sequenced in mussels, Mytilus galloprovincialis. Transcription levels of ovoA in mantle did not significantly change along the reproductive cycle. No alterations of ovoA transcription were observed after a laboratory copper (10 µg/L) exposure or in mussels captured in a highly polluted site. Conversely, the metabolomic analysis of the hydrophilic metabolite content in mantle clearly classified mussels according to their site of origin, especially at the most advanced stages of oogenesis. Quantification of OSH-A and -B and glutathione (GSH), revealed stable levels in mantle at early gametogenesis in the unpolluted sampling site, but a strong increase in female mantle previous to spawning in the polluted site. These increased concentrations under pollution suggest that OSH-A accumulates along oogenesis, independent of gene transcription regulation. The concerted accumulation of OSH-A and GSH suggests the building of a balanced cellular redox-system to scavenge ROS produced in the oocyte before and during fertilization.es_ES
dc.description.sponsorshipBasque Government through project IT1302-19 and Spanish MINECO through project EU-FEDER/ERDF AGL2015-63936-R. B.G.G. is the recipient of a postdoctoral Juan de la Cierva fellowship of the Spanish Ministry of Science and Innovation.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/AGL2015-63936-Res_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/
dc.subjectMytilus galloprovincialis ovothioles_ES
dc.subjectovothiol synthasees_ES
dc.subjectglutathionees_ES
dc.subjectoxidative stresses_ES
dc.subjectmetabolomees_ES
dc.subjectoocyteses_ES
dc.subjectpollution biomonitoringes_ES
dc.titleGametogenesis-Related Fluctuations in Ovothiol Levels in the Mantle of Mussels from Different Estuaries: Fighting Oxidative Stress for Spawning in Polluted Waterses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2020-03-27T14:53:36Z
dc.rights.holder© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)es_ES
dc.relation.publisherversionhttps://www.mdpi.com/2218-273X/10/3/373es_ES
dc.identifier.doi10.3390/biom10030373
dc.departamentoesZoología y biología celular animal
dc.departamentoesQuímica analítica
dc.departamentoeuZoologia eta animalia zelulen biologia
dc.departamentoeuKimika analitikoa


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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
Except where otherwise noted, this item's license is described as © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)