BRAF V600E mutational load as a prognosis biomarker in malignant melanoma
dc.contributor.author | Sevilla Mambrilla, Arrate | |
dc.contributor.author | Morales González, María Celia | |
dc.contributor.author | Ezcurra García Unzueta, Pilar Ariadna | |
dc.contributor.author | Rasero, Javier | |
dc.contributor.author | Velasco, Verónica | |
dc.contributor.author | Cancho Galán, Goikoane | |
dc.contributor.author | Sánchez Díez, Ana | |
dc.contributor.author | Mujika, Karmele | |
dc.contributor.author | Penas Lago, Cristina | |
dc.contributor.author | Smith Zubiaga, Isabel | |
dc.contributor.author | Asumendi Mallea, Aintzane | |
dc.contributor.author | Cortés Díaz, Jesús María | |
dc.contributor.author | Boyano López, María Dolores | |
dc.contributor.author | Alonso Alegre, Santos | |
dc.date.accessioned | 2020-06-11T10:47:40Z | |
dc.date.available | 2020-06-11T10:47:40Z | |
dc.date.issued | 2020-03-13 | |
dc.identifier.citation | Plos One 15(3) : (2020) // Article ID e0230136 | es_ES |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10810/43904 | |
dc.description.abstract | Analyzing the mutational load of driver mutations in melanoma could provide valuable information regarding its progression. We aimed at analyzing the heterogeneity of mutational load of BRAF V600E in biopsies of melanoma patients of different stages, and investigating its potential as a prognosis factor. Mutational load of BRAF V600E was analyzed by digital PCR in 78 biopsies of melanoma patients of different stages and 10 nevi. The BRAF V600E load was compared among biopsies of different stages. Results showed a great variability in the load of V600E (0%-81%). Interestingly, we observed a significant difference in the load of V600E between the early and late melanoma stages, in the sense of an inverse correlation between BRAF V600E mutational load and melanoma progression. In addition, a machine learning approach showed that the mutational load of BRAF V600E could be a good predictor of metastasis in stage II patients. Our results suggest that BRAF V600E is a promising biomarker of prognosis in stage II patients. | es_ES |
dc.description.sponsorship | This research was supported by the Basque Government (grants ELKARTEK-KK2016-036 and KK2017-041 to MDB, grant IT1138-16 to SA and predoctoral fellowship PRE_2014_1_419 to AS), and by the University of the Basque Country (UPV/EHU) (grant GIU17/066). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library Science | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | digital PCR | es_ES |
dc.title | BRAF V600E mutational load as a prognosis biomarker in malignant melanoma | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | 2020 Sevilla et al. This is an open access article distributed under the terms of the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230136 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0230136 | |
dc.departamentoes | Biología celular e histología | es_ES |
dc.departamentoes | Genética, antropología física y fisiología animal | es_ES |
dc.departamentoes | Zoología y biología celular animal | es_ES |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia | es_ES |
dc.departamentoeu | Zelulen biologia eta histologia | es_ES |
dc.departamentoeu | Zoologia eta animalia zelulen biologia | es_ES |
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Except where otherwise noted, this item's license is described as 2020 Sevilla et al. This is an open access article distributed under the terms of the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.