Overcoming the Inflammatory Stage of Non-Healing Wounds: In Vitro Mechanism of Action of Negatively Charged Microspheres (NCMs)
dc.contributor.author | Santos Vizcaíno, Edorta ![]() | |
dc.contributor.author | Salvador Martínez, Aiala ![]() | |
dc.contributor.author | Vairo, Claudia | |
dc.contributor.author | Igartua Olaechea, Manuela ![]() | |
dc.contributor.author | Hernández Martín, Rosa María ![]() | |
dc.contributor.author | Correa, Luis | |
dc.contributor.author | Villullas, Silvia | |
dc.contributor.author | Gainza Luzea, Garazi | |
dc.date.accessioned | 2020-07-02T12:10:36Z | |
dc.date.available | 2020-07-02T12:10:36Z | |
dc.date.issued | 2020-06-03 | |
dc.identifier.citation | Nanomaterials 10(6) : (2020) // Article ID 1108 | es_ES |
dc.identifier.issn | 2079-4991 | |
dc.identifier.uri | http://hdl.handle.net/10810/44970 | |
dc.description.abstract | Negatively charged microspheres (NCMs) represent a new therapeutic approach for wound healing since recent clinical trials have shown NCM efficacy in the recovery of hard-to-heal wounds that tend to stay in the inflammatory phase, unlocking the healing process. The aim of this study was to elucidate the NCM mechanism of action. NCMs were extracted from a commercial microsphere formulation (PolyHeal® Micro) and cytotoxicity, attachment, proliferation and viability assays were performed in keratinocytes and dermal fibroblasts, while macrophages were used for the phagocytosis and polarization assays. We demonstrated that cells tend to attach to the microsphere surface, and that NCMs are biocompatible and promote cell proliferation at specific concentrations (50 and 10 NCM/cell) by a minimum of 3 fold compared to the control group. Furthermore, NCM internalization by macrophages seemed to drive these cells to a noninflammatory condition, as demonstrated by the over-expression of CD206 and the under-expression of CD64, M2 and M1 markers, respectively. NCMs are an effective approach for reverting the chronic inflammatory state of stagnant wounds (such as diabetic wounds) and thus for improving wound healing. | es_ES |
dc.description.sponsorship | The present work was partially funded by the Basque Government (Consolidated Groups, IT-907-16 and HAZITEK, ZE-2017/00014) and co-funded by the European Regional Development Fund (ERDF). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | chronic wound | es_ES |
dc.subject | device | es_ES |
dc.subject | foot ulcer | es_ES |
dc.subject | inflammation | es_ES |
dc.subject | wound healing | es_ES |
dc.subject | macrophage | es_ES |
dc.title | Overcoming the Inflammatory Stage of Non-Healing Wounds: In Vitro Mechanism of Action of Negatively Charged Microspheres (NCMs) | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2020-06-30T16:27:23Z | |
dc.rights.holder | 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2079-4991/10/6/1108/htm | es_ES |
dc.identifier.doi | 10.3390/nano10061108 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | |
dc.departamentoeu | Farmazia eta elikagaien zientziak |
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Except where otherwise noted, this item's license is described as 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).