dc.contributor.author | Prieto Fernández, Endika | |
dc.contributor.author | Aransay Bañares, Ana María | |
dc.contributor.author | Royo, Félix | |
dc.contributor.author | González Jiménez, María Esperanza | |
dc.contributor.author | Lozano, Juan José | |
dc.contributor.author | Santos Zorrozúa, Borja ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Macías Cámara, Nuria | |
dc.contributor.author | González López, Monika | |
dc.contributor.author | Pérez Garay, Raquel | |
dc.contributor.author | Benito Fernández, Francisco Javier | |
dc.contributor.author | García-Orad Carles, África ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Falcón Pérez, Juan Manuel | |
dc.date.accessioned | 2020-12-17T13:10:58Z | |
dc.date.available | 2020-12-17T13:10:58Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Theranostics 9(16) : 4567-4579 (2019) | es_ES |
dc.identifier.issn | 1838-7640 | |
dc.identifier.uri | http://hdl.handle.net/10810/49143 | |
dc.description.abstract | Cerebrospinal fluid (CSF) microRNAs (miRNAs) have emerged as potential biomarkers for minimally invasive diagnosis of central nervous system malignancies. However, despite significant advances in recent years, this field still suffers from poor data reproducibility. This is especially true in cases of infants, considered a new subject group. Implementing efficient methods to study miRNAs from clinically realistic CSF volumes is necessary for the identification of new biomarkers.
Methods: We compared six protocols for characterizing miRNAs, using 200-mu L CSF from infants (aged 0-7). Four of the methods employed extracellular vesicle (EV) enrichment step and the other two obtained the miRNAs directly from cleared CSF. The efficiency of each method was assessed using real-time PCR and small RNA sequencing. We also determined the distribution of miRNAs among different CSF shuttles, using size-exclusion chromatography.
Results: We identified 281 CSF miRNAs from infants. We demonstrated that the miRNAs could be efficiently detected using only 200 mu L of biofluid in case of at least two of the six methods. In the exosomal fraction, we found 12 miRNAs that might be involved in neurodevelopment.
Conclusion: The Norgen and Invitrogen protocols appear suitable for the analysis of a large number of miRNAs using small CSF samples. | es_ES |
dc.description.sponsorship | This work was supported by the Basque Government [IT989-16], the Spanish Ministry of Economy and Competitiveness MINECO [SAF2015066312], and the Ramon Areces Foundation [FRA-17-JMF]. We thank MINECO for the REDIEX (Spanish Excellence Network in Exosomes) and the Severo Ochoa Excellence Accreditation (SEV-2016-0644). Funding for open access charge: Severo Ochoa Excellence Accreditation (SEV-2016-0644). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Ivyspring International Publisher | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2015066312 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | CSF miRNAs | es_ES |
dc.subject | CSF exosomes | es_ES |
dc.subject | microRNA profiling | es_ES |
dc.subject | infants | es_ES |
dc.subject | clinical samples traumatic brain-injury | es_ES |
dc.subject | differential expression | es_ES |
dc.subject | extracellular vesicles | es_ES |
dc.subject | microrna | es_ES |
dc.subject | biomarker | es_ES |
dc.subject | disease | es_ES |
dc.subject | exosome | es_ES |
dc.subject | visualization | es_ES |
dc.subject | glioblastoma | es_ES |
dc.subject | diagnosis | es_ES |
dc.title | A Comprehensive Study of Vesicular and Non-Vesicular miRNAs from a Volume of Cerebrospinal Fluid Compatible with Clinical Practice | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.thno.org/v09p4567.htm | es_ES |
dc.identifier.doi | 10.7150/thno.31502 | |
dc.departamentoes | Genética, antropología física y fisiología animal | es_ES |
dc.departamentoes | Pediatría | es_ES |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia | es_ES |
dc.departamentoeu | Pediatria | es_ES |