Lack of the transient receptor potential vanilloid 1 shifts cannabinoid-dependent excitatory synaptic plasticity in the dentate gyrus of the mouse brain hippocampus
dc.contributor.author | Egaña Huguet, Jon | |
dc.contributor.author | Saumell Esnaola, Miquel | |
dc.contributor.author | Achicallende Urcaregui, Svein | |
dc.contributor.author | Soria Gómez, Edgar | |
dc.contributor.author | Bonilla del Río, Itziar | |
dc.contributor.author | García del Caño, Gontzal | |
dc.contributor.author | Barrondo Lacarra, Sergio | |
dc.contributor.author | Sallés Alvira, Joan | |
dc.contributor.author | Gerrikagoitia Marina, Inmaculada | |
dc.contributor.author | Puente Bustinza, Nagore | |
dc.contributor.author | Elezgarai Gabantxo, Izaskun | |
dc.contributor.author | Grandes Moreno, Pedro Rolando | |
dc.date.accessioned | 2021-08-04T12:09:37Z | |
dc.date.available | 2021-08-04T12:09:37Z | |
dc.date.issued | 2021-07-07 | |
dc.identifier.citation | Frontiers in Neuroanatomy 15 : (2021) // Article ID 701573 | es_ES |
dc.identifier.issn | 1662-5129 | |
dc.identifier.uri | http://hdl.handle.net/10810/52659 | |
dc.description.abstract | [EN] The transient receptor potential vanilloid 1 (TRPV1) participates in synaptic functions in the brain. In the dentate gyrus, post-synaptic TRPV1 in the granule cell (GC) dendritic spines mediates a type of long-term depression (LTD) of the excitatory medial perforant path (MPP) synapses independent of pre-synaptic cannabinoid CB1 receptors. As CB1 receptors also mediate LTD at these synapses, both CB1 and TRPV1 might be influencing the activity of each other acting from opposite synaptic sites. We tested this hypothesis in the MPP–GC synapses of mice lacking TRPV1 (TRPV1-/-). Unlike wild-type (WT) mice, low-frequency stimulation (10min at 10Hz) of TRPV1-/- MPP fibers elicited a form of long-term potentiation (LTP) that was dependent on (1) CB1 receptors, (2) the endocannabinoid 2-arachidonoylglycerol (2-AG), (3) rearrangement of actin filaments, and (4) nitric oxide signaling. These functional changes were associated with an increase in the maximum binding efficacy of guanosine-5′-O-(3-[35S]thiotriphosphate) ([35S]GTPgS) stimulated by the CB1 receptor agonist CP 55,940, and a significant decrease in receptor basal activation in the TRPV1-/- hippocampus. Finally, TRPV1-/- hippocampal synaptosomes showed an augmented level of the guanine nucleotide-binding (G) Gai1, Gai2, and Gai3 protein alpha subunits. Altogether, the lack of TRPV1 modifies CB1 receptor signaling in the dentate gyrus and causes the shift from CB1 receptor-mediated LTD to LTP at the MPP–GC synapses. | es_ES |
dc.description.sponsorship | This work was supported by the Basque Government (IT1230- 19, to PG); MINECO/FEDER, UE (SAF2015-65034-R, to PG); Ministry of Science and Innovation (PID2019-107548RBI00, to PG); Red de Trastornos Adictivos, Instituto de Salud Carlos III (ISC-III); and European Regional Development Funds-European Union (ERDF-EU, Investing in your future; RD16/0017/0012, to PG); MINECO CTQ2017-85686-R (Spanish Ministry of Economy and Competitiveness, to JS); JE-H is a Postdoctoral Researcher contracted with funds of Red de Trastornos Adictivos, Instituto de Salud Carlos III (ISC-III), and European Regional Development Funds-European Union (ERDF-EU, Investing in your future; RD16/0017/0012), and the Basque Government (IT1230-19). IB-D holds a Postdoctoral Orientation Period contract (BES-2016-076766, BES-C-2016-0051). SA has a Ph.D. contract granted by University of the Basque Country (PIF 16/251). ES-G is funded by Ikerbasque and MINECO (PGC2018- 093990-A-I00; MICIU/AEI/FEDER, UE). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers Media | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2015-65034-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/PID2019-107548RBI00 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/CTQ2017-85686-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/PGC2018-093990-A-I00 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | endovanilloid system | es_ES |
dc.subject | CB1 receptor | es_ES |
dc.subject | excitatory synapses | es_ES |
dc.subject | long-term potentiation | es_ES |
dc.subject | G proteins | es_ES |
dc.title | Lack of the transient receptor potential vanilloid 1 shifts cannabinoid-dependent excitatory synaptic plasticity in the dentate gyrus of the mouse brain hippocampus | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2021 Egaña-Huguet, Saumell-Esnaola, Achicallende, Soria-Gomez, Bonilla-Del Río, García del Caño, Barrondo, Sallés, Gerrikagoitia, Puente, Elezgarai and Grandes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fnana.2021.701573 | es_ES |
dc.identifier.doi | 10.3389/fnana.2021.701573 | |
dc.departamentoes | Farmacología | es_ES |
dc.departamentoes | Neurociencias | es_ES |
dc.departamentoeu | Farmakologia | es_ES |
dc.departamentoeu | Neurozientziak | es_ES |
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