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dc.contributor.authorRomero Gavilán, Francisco
dc.contributor.authorCerqueira, Andreia
dc.contributor.authorAnitua Aldekoa, Eduardo
dc.contributor.authorTejero, Ricardo
dc.contributor.authorGarcía Arnáez, Iñaki
dc.contributor.authorMartínez Ramos, Cristina
dc.contributor.authorOzturan, Seda
dc.contributor.authorIzquierdo, Raúl
dc.contributor.authorAzkargorta, Mikel
dc.contributor.authorElortza, Felix
dc.contributor.authorGurruchaga Torrecilla, María Dolores ORCID
dc.contributor.authorGoñi Echave, Isabel María del Coro ORCID
dc.contributor.authorSuay, Julio
dc.date.accessioned2021-10-22T12:26:58Z
dc.date.available2021-10-22T12:26:58Z
dc.date.issued2021-08-27
dc.identifier.citationJournal Of Biological Inorganic Chemistry 26(6) : 715-726 (2021)es_ES
dc.identifier.issn0949-8257
dc.identifier.issn1432-1327
dc.identifier.urihttp://hdl.handle.net/10810/53531
dc.description.abstractCalcium ions are used in the development of biomaterials for the promotion of coagulation, bone regeneration, and implant osseointegration. Upon implantation, the time-dependent release of calcium ions from titanium implant surfaces modifies the physicochemical characteristics at the implant-tissue interface and thus, the biological responses. The aim of this study is to examine how the dynamics of protein adsorption on these surfaces change over time. Titanium discs with and without Ca were incubated with human serum for 2 min, 180 min, and 960 min. The layer of proteins attached to the surface was characterised using nLC-MS/MS. The adsorption kinetics was different between materials, revealing an increased adsorption of proteins associated with coagulation and immune responses prior to Ca release. Implant-blood contact experiments confirmed the strong coagulatory effect for Ca surfaces. We employed primary human alveolar osteoblasts and THP-1 monocytes to study the osteogenic and inflammatory responses. In agreement with the proteomic results, Ca-enriched surfaces showed a significant initial inflammation that disappeared once the calcium was released. The distinct protein adsorption/desorption dynamics found in this work demonstrated to be useful to explain the differential biological responses between the titanium and Ca-ion modified implant surfaces. Graphic abstractes_ES
dc.description.sponsorshipThis work was supported by MINECO [MAT2017-86043-R; RTC-2017-6147-1], Generalitat Valenciana [GRISOLIAP/2018/091; APOSTD/2020/036, PROMETEO/2020/069], Universitat Jaume I under [ UJI-B2017-37], the University of the Basque Country under [GIU18/189] and Basque Government under [PRE_2017_2_0044]es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/MAT2017-86043-Res_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/RTC-2017-6147-1es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectproteomicses_ES
dc.subjectbioinorganic chemistryes_ES
dc.subjectdental implantses_ES
dc.subjectosseointegrationes_ES
dc.subjectblood clottinges_ES
dc.subjectosteogenic differentiationes_ES
dc.subjectproteomic analysises_ES
dc.subjectimplant surfaceses_ES
dc.subjectgrowth-factorses_ES
dc.subjectplasma riches_ES
dc.subjectcalciumes_ES
dc.subjectionses_ES
dc.subjectbiomaterialses_ES
dc.subjectinflammationes_ES
dc.subjectcoagulationes_ES
dc.titleProtein adsorption/desorption dynamics on Ca-enriched titanium surfaces: biological implicationses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderThis article is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0)es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://link-springer-com.ehu.idm.oclc.org/article/10.1007%2Fs00775-021-01886-4es_ES
dc.identifier.doi10.1007/s00775-021-01886-4
dc.departamentoesPolímeros y Materiales Avanzados: Física, Química y Tecnologíaes_ES
dc.departamentoeuPolimero eta Material Aurreratuak: Fisika, Kimika eta Teknologiaes_ES


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