A reproducible method for biochemical, histological and functional assessment of the effects of ischaemia–reperfusion syndrome in the lower limbsIñig
dc.contributor.author | Cearra Guezuraga, Iñigo | |
dc.contributor.author | Herrero de la Parte, Borja | |
dc.contributor.author | Moreno Franco, Diana Isabel | |
dc.contributor.author | García-Alonso Montoya, Ignacio | |
dc.date.accessioned | 2021-11-16T08:38:02Z | |
dc.date.available | 2021-11-16T08:38:02Z | |
dc.date.issued | 2021-09-29 | |
dc.identifier.citation | Scientific Reports 11(1) : (2021) // Article ID 19325 | es_ES |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10810/53763 | |
dc.description.abstract | [EN]Current methodology described to mimic lower limb ischaemia-reperfusion injury (LL-IRI) does not accurately define the procedures and pressures exerted to induce and maintain ischaemia. In this piece of work, we propose a well-defined and detailed rat model that simulates the conditions established in clinical practice guidelines for tourniquet application and allows us to test treatments that aim to prevent/reduce LL-IRI. Eighty-six male WAG/RijHsd rats were subjected to hind limb IRI (LL-IRI), using a mechanical system applying a 1 kg tension to induce and maintain ischemia for 2 or 3 h, and assessed the damage caused by reperfusion at biochemical and muscular levels at different time points. At the biochemical level, both 2 and 3 h of ischemia induced changes (except for electrolyte levels); 3 h of ischemia induced greater changes in specific markers of muscular damage: creatine kinase (CK) and lactate dehydrogenase (LDH). At the histopathological level, 3 h of ischemia and 24 h of reperfusion was associated with an increase in hind limb girth, cross-sectional area, and weight and presence of neutrophils, as well as histological damage in more than 60% of muscle fibres. Our model allows to reliably reproduce the damage associated with the use of a pneumatic tourniquet. CK and LDH, as well as measures of tissue damage, allow to define and characterize the response to LL-IRI-related damage. A period of 3 h of ischemia followed by 3 h of reperfusion caused only local damage but showed greater sensitivity to detect differences in future studies on prophylactic treatments against LL-IRI. | es_ES |
dc.description.sponsorship | No external funding was received. This study has been supported by internal funding from the Department of the Surgery, Radiology and Physical Medicine at the University of the Basque Country (UPV/EHU). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Research | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | skeletal-muscle ischemia | es_ES |
dc.subject | tourniquet use | es_ES |
dc.subject | extremity surgery | es_ES |
dc.subject | folinic acid | es_ES |
dc.subject | injury | es_ES |
dc.subject | propofol | es_ES |
dc.subject | tolerance | es_ES |
dc.subject | integrity | es_ES |
dc.subject | melatonin | es_ES |
dc.subject | severity | es_ES |
dc.title | A reproducible method for biochemical, histological and functional assessment of the effects of ischaemia–reperfusion syndrome in the lower limbsIñig | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/. © The Author(s) 2021 | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-021-98887-9 | es_ES |
dc.identifier.doi | 10.1038/s41598-021-98887-9 | |
dc.departamentoes | Cirugía, radiología y medicina física | es_ES |
dc.departamentoeu | Kirurgia,erradiologia eta medikuntza fisikoa | es_ES |
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© The Author(s) 2021