Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model
dc.contributor.author | Báez Díaz, Claudia | |
dc.contributor.author | Blanco Blázquez, Virginia | |
dc.contributor.author | Sánchez-Margallo, Francisco Miguel | |
dc.contributor.author | López, Esther | |
dc.contributor.author | Martín, Helena | |
dc.contributor.author | Espona Noguera, Albert | |
dc.contributor.author | Casado, Javier G. | |
dc.contributor.author | Ciriza, Jesús | |
dc.contributor.author | Pedraz Muñoz, José Luis ![]() | |
dc.contributor.author | Crisóstomo, Verónica | |
dc.date.accessioned | 2021-12-01T11:03:36Z | |
dc.date.available | 2021-12-01T11:03:36Z | |
dc.date.issued | 2021-11-01 | |
dc.identifier.citation | Pharmaceutics 13(11) : (2021) // Article ID 1824 | es_ES |
dc.identifier.issn | 1999-4923 | |
dc.identifier.uri | http://hdl.handle.net/10810/54247 | |
dc.description.abstract | The administration of cardiosphere-derived cells (CDCs) after acute myocardial infarction (AMI) is very promising. CDC encapsulation in alginate-poly-l-lysine-alginate (APA) could increase cell survival and adherence. The intrapericardial (IP) approach potentially achieves high concentrations of the therapeutic agent in the infarcted area. We aimed to evaluate IP therapy using a saline vehicle as a control (CON), a dose of 30 × 106 CDCs (CDCs) or APA microcapsules containing 30 × 106 CDCs (APA-CDCs) at 72 h in a porcine AMI model. Magnetic resonance imaging (MRI) was used to determine the left ventricular ejection fraction (LVEF), infarct size (IS), and indexed end diastolic and systolic volumes (EDVi; ESVi) pre- and 10 weeks post-injection. Programmed electrical stimulation (PES) was performed to test arrhythmia inducibility before euthanasia. Histopathological analysis was carried out afterwards. The IP infusion was successful in all animals. At 10 weeks, MRI revealed significantly higher LVEF in the APA-CDC group compared with CON. No significant differences were observed among groups in IS, EDVi, ESVi, PES and histopathological analyses. In conclusion, the IP injection of CDCs (microencapsulated or not) was feasible and safe 72 h post-AMI in the porcine model. Moreover, CDCs APA encapsulation could have a beneficial effect on cardiac function, reflected by a higher LVEF at 10 weeks. | es_ES |
dc.description.sponsorship | This research was funded by the Agencia Estatal de Investigación (PID2019-107329RA-C22/AEI/10.13039/501100011033), the Consejería de Economía, Ciencia y Agenda Digital, Junta de Extremadura (IB20184, IB20191, GR18199) and the Instituto de Salud Carlos III (PI20/00247, CB16/11/00494 and Sara Borrell CD19/00048), and cofunded by the European Regional Development Fund “A way to make Europe”. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | CDCs | es_ES |
dc.subject | AM | es_ES |
dc.subject | intrapericardial | es_ES |
dc.subject | microcapsules | es_ES |
dc.subject | APA | es_ES |
dc.subject | swine | es_ES |
dc.title | Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-11-25T16:00:15Z | |
dc.rights.holder | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1999-4923/13/11/1824 | es_ES |
dc.identifier.doi | 10.3390/pharmaceutics13111824 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | |
dc.departamentoeu | Farmazia eta elikagaien zientziak |
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Except where otherwise noted, this item's license is described as 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).