Effects of Folinic Acid Administration on Lower Limb Ischemia/Reperfusion Injury in Rats
dc.contributor.author | Cearra Guezuraga, Iñigo | |
dc.contributor.author | Herrero de la Parte, Borja | |
dc.contributor.author | Ruiz Montesinos, María Inmaculada ![]() | |
dc.contributor.author | Alonso Varona, Ana Isabel | |
dc.contributor.author | Moreno Franco, Diana Isabel | |
dc.contributor.author | García-Alonso Montoya, Ignacio ![]() | |
dc.date.accessioned | 2021-12-28T09:45:22Z | |
dc.date.available | 2021-12-28T09:45:22Z | |
dc.date.issued | 2021-11-25 | |
dc.identifier.citation | Antioxidants 10(12) : (2021) // Article ID 1887 | es_ES |
dc.identifier.issn | 2076-3921 | |
dc.identifier.uri | http://hdl.handle.net/10810/54749 | |
dc.description.abstract | Surgery under ischemic conditions, lasting up to 3 h, is routinely performed in orthopedic surgery, causing undesirable injury due to ischemia-reperfusion syndrome, with short and medium-term functional repercussions. To date, there is no established prophylactic treatment. In this work we evaluated folinic acid (FA) in a rodent model of lower limb ischemia-reperfusion (IRI-LL). 36 male WAG rats underwent 3 h of lower limb ischemia. In the saline group, rats received intraperitoneal administration of saline (used as vehicle for treatment). In the experimental group, rats were pretreated with FA (2.5 mg/kg) before the end of ischemia. After ischemia, animals were sacrificed at 3 h, 24 h or 14 days (for biochemical determination (Na+, K+, Cl-, urea, creatinine, CK, LDH, ALP, ALT, and AST), pathological assessment, or functional study using the rotarod test; respectively). Another six animals were used to establish the reference values. The prophylactic administration of FA significantly reduced the elevation of biochemical markers, especially those that most directly indicate muscle damage (CK and LDH). In addition, it also improved direct tissue damage, both in terms of edema, weight, PMN infiltrate and percentage of damaged fibers. Finally, the administration of FA allowed the animals to equal baseline values in the rotarod test; what did not occur in the saline group, where pre-ischemia levels were not recovered. Following 3 h of lower limb ischemia, FA minimizes the increase of CK and LDH, as well as local edema and leukocyte infiltration, allowing a faster recovery of limb functionality. Therefore, it could be considered as a prophylactic treatment when tourniquet is used in clinics. | es_ES |
dc.description.sponsorship | This research received funding from the University of The Basque Country UPV/EHU (grant reference GIU19/088). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.title | Effects of Folinic Acid Administration on Lower Limb Ischemia/Reperfusion Injury in Rats | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-12-23T15:06:33Z | |
dc.rights.holder | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2076-3921/10/12/1887 | es_ES |
dc.identifier.doi | 10.3390/antiox10121887 | |
dc.departamentoes | Cirugía, radiología y medicina física | |
dc.departamentoes | Biología celular e histología | |
dc.departamentoeu | Kirurgia,erradiologia eta medikuntza fisikoa | |
dc.departamentoeu | Zelulen biologia eta histologia |
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Except where otherwise noted, this item's license is described as © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).