BackCryo-EM structure of translesion DNA synthesis polymerase ζ with a base pair mismatch
| dc.contributor.author | Malik, Radhika | |
| dc.contributor.author | Johnson, Robert E. | |
| dc.contributor.author | Prakash, Louise | |
| dc.contributor.author | Prakash, Satya | |
| dc.contributor.author | Ubarrechena Belandia, Iván  | |
| dc.contributor.author | Aggarwal, Aneel K. | |
| dc.date.accessioned | 2022-03-03T08:38:04Z | |
| dc.date.available | 2022-03-03T08:38:04Z | |
| dc.date.issued | 2022-02 | |
| dc.identifier.citation | Nature Communications 13(1) : (2022) // Article ID 1050 | es_ES |
| dc.identifier.issn | 2041-1723 | |
| dc.identifier.uri | http://hdl.handle.net/10810/55650 | |
| dc.description.abstract | [EN] The B-family multi-subunit DNA polymerase ζ (Polζ) is important for translesion DNA synthesis (TLS) during replication, due to its ability to extend synthesis past nucleotides opposite DNA lesions and mismatched base pairs. We present a cryo-EM structure of Saccharomyces cerevisiae Polζ with an A:C mismatch at the primer terminus. The structure shows how the Polζ active site responds to the mismatched duplex DNA distortion, including the loosening of key protein-DNA interactions and a fingers domain in an “open” conformation, while the incoming dCTP is still able to bind for the extension reaction. The structure of the mismatched DNA-Polζ ternary complex reveals insights into mechanisms that either stall or favor continued DNA synthesis in eukaryotes. | es_ES |
| dc.description.sponsorship | This work was funded by grants R01-GM124047 (A.K.A and L.P) and R35-GM13170 (A.K.A) from the National Institutes of Health (NIH). I.U.-B was supported by a grant PID2019-104423GB-I00/AEI/10.13039/501100011033 from the Spanish State Research Agency and by the Basque Excellence Research Centre program. Most of the cryo-EM work was performed at the Simons Electron Microscopy Center and National Resource for Automated Molecular Microscopy, located at the New York Structural Biology Center, supported by grants from the Simons Foundation (SF349247), NYSTAR, and the NIH National Institute of General Medical Sciences (GM103310), with additional support from Agouron Institute (F00316), NIH (OD019994) and NIH (RR029300). Computing resources needed for this work were provided in part by the High Performance Computing facility of the Icahn School of Medicine at Mount Sinai. Molecular graphics and analyses were performed with UCSF Chimera, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from NIH P41-GM103311. | es_ES |
| dc.language.iso | eng | es_ES |
| dc.publisher | Nature Research | es_ES |
| dc.relation | info:eu-repo/grantAgreement/MICINN/PID2019-104423GB-I00 | es_ES |
| dc.rights | info:eu-repo/semantics/openAccess | es_ES |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
| dc.title | Cryo-EM structure of translesion DNA synthesis polymerase ζ with a base pair mismatch | es_ES |
| dc.type | info:eu-repo/semantics/article | es_ES |
| dc.rights.holder | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | es_ES |
| dc.rights.holder | Atribución 3.0 España | * |
| dc.relation.publisherversion | https://www.nature.com/articles/s41467-022-28644-7 | es_ES |
| dc.identifier.doi | 10.1038/s41467-022-28644-7 |
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