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dc.contributor.authorAcera Osa, Arantxa
dc.contributor.authorAbad García, Beatriz
dc.contributor.authorPereiro Díez, Xandra ORCID
dc.contributor.authorRodríguez, Francisco David
dc.contributor.authorRuzafa Andrés, Noelia ORCID
dc.contributor.authorDurán de la Colina, Juan Antonio
dc.contributor.authorVecino Cordero, Elena ORCID
dc.identifier.citationExperimental Eye Research 219 : (2022) // Article ID 109061es_ES
dc.description.abstract[EN] The Tear Film Lipid Layer (TFLL) acts primarily as an interface between the aqueous layer and air. Tear film lipid is composed of a thin layer of polar lipids that interact with the secretory layer of the underlying mucosa and a thicker layer of non-polar lipids at the air interface. The tear film has a complex structure and composition that protects the cornea, promotes wound healing, and maintains high-quality vision. Plasma Rich in Growth Factor (PRGF) eye drops emerged as an exciting new treatment for corneal epitheliopathies, including aqueous deficient dry eye. The purpose of this study was to compare the lipidomic profile of eye drops obtained from PRGF with tear lipidome to determine whether PRGF drops could be an adequate complement to tears in patients with impaired TFLL. To address this study, tears and blood was collected and processed from healthy donors to obtain PRGF eye drops. Samples were aliquoted and stored at -80°C until use. The lipid profiles of these samples were analysed by Ultrahigh Performance Liquid Chromatography (UHPLC) using a Vanquish UHPLC system to obtain untargeted lipidome profiles on a Q-Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer. In PRGF eye drops, 408 lipids were identified in ESI+mode and 183 in ESI- mode, and they were grouped into 15 different lipid classes from four distinct categories. By contrast, 112 lipid species were identified from tear samples in ESI+mode and 36 in ESI- mode, belonging to 12 lipid classes from six different categories. The relative abundance of most lipid species was much greater in the PRGF eye drops than in the tear, although there were some lipids present in tears that were not found in the PRGF, such as wax esters and (O-acyl)-omega-hydroxy fatty acids. In summary, these results suggest that the lipids present in PRGF eye drops could serve as a tear supplement in individuals in whom tear lipid composition is altered, although there are differences in the lipid profile of these two fluids.es_ES
dc.description.sponsorshipThe authors wish to acknowledge the financial support received to carry out this work from: MINECO-Retos Fondos Fender (RTC-2016-48231), Gobierno Vasco (PUE_2018_1_0004), ELKARTEK (KK-2019/00086), PIBA 2020-1-0026, MINECO-Retos (PID2019-111139RB-I00), ELKARTEK (KK-2021-00023) to EV and FISS-21-RD21/0002/0041 to AA.es_ES
dc.subjecttear filmes_ES
dc.subjectPRGF eye dropses_ES
dc.subjectdry eyees_ES
dc.subjecttear film lipid layeres_ES
dc.titleComparative study of the lipid profile of tears and plasma enriched in growth factors.es_ES
dc.rights.holder© 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (
dc.rights.holderAtribución-NoComercial-SinDerivadas 3.0 España*
dc.departamentoesBiología celular e histologíaes_ES
dc.departamentoesDermatología, oftalmología y otorrinolaringologíaes_ES
dc.departamentoeuDermatologia, oftalmologia eta otorrinolaringologiaes_ES
dc.departamentoeuZelulen biologia eta histologiaes_ES

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© 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (
Except where otherwise noted, this item's license is described as © 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (