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Arcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formation

dc.contributor.authorSalazar Sánchez, Adrián
dc.contributor.authorBaztarrika, Itsaso
dc.contributor.authorAlonso Monsalve, Rodrigo
dc.contributor.authorFernández Astorga, Aurora ORCID
dc.contributor.authorMartínez Ballesteros, Ilargi ORCID
dc.contributor.authorMartínez Malax-Echevarría, Irati ORCID
dc.date.accessioned2022-08-05T09:35:40Z
dc.date.available2022-08-05T09:35:40Z
dc.date.issued2022
dc.identifier.citationMicroorganisms 10(7) : (2022) // Article ID 1280es_ES
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10810/57222
dc.description.abstractArcobacter butzleri, the most prevalent species of the genus, has the demonstrated ability to adhere to various surfaces through biofilm production. The biofilm formation capability has been related to the expression of certain genes, which have not been characterized in A. butzleri. In order to increase the knowledge of this foodborne pathogen, the aim of this study was to assess the role of six biofilm-associated genes in campylobacteria (flaA, flaB, fliS, luxS, pta and spoT) in the biofilm formation ability of A. butzleri. Knockout mutants were constructed from different foodborne isolates, and static biofilm assays were conducted on polystyrene (PS), reinforced glass and stainless steel. Additionally, motility and Congo red binding assays were performed. In general, mutants in flaAB, fliS and luxS showed a decrease in the biofilm production irrespective of the surface; mutants in spoT showed an increase on stainless steel, and mutants in pta and spoT showed a decrease on reinforced glass but an increase on PS. Our work sheds light on the biofilm-related pathogenesis of A. butzleri, although future studies are necessary to achieve a satisfactory objective.es_ES
dc.description.sponsorshipThis research was funded by the Spanish Ministry of Economy and Competitiveness, grant number AGL2014-56179-P (co-financed with FEDER funds), the University of the Basque Country UPV/EHU, grant number PPG17/27, and by the Basque Government, grant number PA20/03. A.S.-S. received a Ph.D. fellowship from the University of the Basque Country UPV/EHU, and I.B from the Basque Government.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/AGL2014-56179-Pes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectArcobacter butzleries_ES
dc.subjectbiofilmes_ES
dc.subjectknockout mutantses_ES
dc.subjectCongo red assayes_ES
dc.subjectstatic biofilm assayses_ES
dc.titleArcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formationes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2022-07-25T16:33:07Z
dc.rights.holder© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).es_ES
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/10/7/1280es_ES
dc.identifier.doi10.3390/microorganisms10071280
dc.departamentoesInmunología, microbiología y parasitología
dc.departamentoeuImmunologia, mikrobiologia eta parasitologia


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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Except where otherwise noted, this item's license is described as © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).