dc.contributor.author | Rodríguez Gallego, María Victoria | |
dc.contributor.author | Aparicio Prieto, Mª Victoria | |
dc.contributor.author | Leza García, Andrea | |
dc.contributor.author | Hernández Hernández, Juana | |
dc.contributor.author | Arizaleta Uralde, José Antonio | |
dc.contributor.author | Casis Sáenz, Luis | |
dc.date.accessioned | 2022-11-23T18:26:51Z | |
dc.date.available | 2022-11-23T18:26:51Z | |
dc.date.issued | 2022-10 | |
dc.identifier.citation | International Journal of Reproductive Biomedicine 20(10) : 851-860 (2022) | es_ES |
dc.identifier.issn | 2476-4108 | |
dc.identifier.issn | 2476-3772 | |
dc.identifier.uri | http://hdl.handle.net/10810/58519 | |
dc.description.abstract | Background: The relationship between the biochemical characteristics of follicular fluid (FF), oocyte quality and embryonic development has not yet been elucidated. We compared samples of FF with a normal metabolic profile against samples with metabolic abnormalities to identify potential predictive biomarkers of reproductive success.
Objective: To analyze peptide activity in the FF of women undergoing in vitro fertilization using 3 samples of FF per individual.
Materials and Methods: FF samples were obtained by ovum pick-up. Pathological samples were defined as samples of FF obtained from women with a gynecological condition or with infertility. A total of 30 women participated in this study. 3 samples of FF were obtained per individual (90 samples), but 8 samples were excluded because they were hemolyzed. The samples (n = 82 FF) included controls (n = 36, donors without fertility problems), women with endometriosis (n = 15), unexplained infertility (n = 19), and aged > 39 (n = 12). We assessed local encephalinergics: aminopeptidase-N (puromycin sensitive aminopeptidase and neutral endopeptidase; and components of the angiotensin system of the reproductive tract: prolyl-endopeptidase, APN, aspartate-aminopeptidase, and basic-aminopeptidase.
Results: No differences were observed in peptide metabolism based on the presence or absence of oocytes in the FF. Women with endometriosis and aged > 39 yr showed alterations in puromycin sensitive aminopeptidase (p = 0.01), aminopeptidase-B (p = 0.01), aspartate-aminopeptidase (p < 0.001) and neutral endopeptidase (p < 0.001).
Conclusion: This study reveals alterations in the metabolism of enkephalin and angiotensin in pathological FF, which points to these components as potential diagnostic biomarkers. | es_ES |
dc.description.sponsorship | This work was supported by
grants from the University of the Basque Country
(UPV/EHU GIU 17/19) and the Gangoiti Barrera
Foundation, Spain (Basque Country). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Shahid Sadoughi University of Medical Sciences | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/es/ | * |
dc.subject | endometriosis | es_ES |
dc.subject | infertility | es_ES |
dc.subject | ovarian diseases | es_ES |
dc.subject | peptides | es_ES |
dc.subject | women | es_ES |
dc.subject | follicular fluid | es_ES |
dc.title | Metabolism of renin-angiotensin and enkephalin in human follicular fluid: An experimental study. | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. | es_ES |
dc.rights.holder | Atribución-NoComercial 3.0 España | * |
dc.relation.publisherversion | https://ijrm.ssu.ac.ir/article-1-2354-en.html | es_ES |
dc.identifier.doi | 10.18502/ijrm.v20i10.12269 | |
dc.departamentoes | Fisiología | es_ES |
dc.departamentoeu | Fisiologia | es_ES |