Development and validation of a new multiplex for upgrading Y-STRs population databases from 12 to 23 markers and its forensic casework application.
dc.contributor.author | Navarro López, Belén | |
dc.contributor.author | Baeta Bafalluy, Miriam ![]() | |
dc.contributor.author | Granizo Rodríguez, Eva | |
dc.contributor.author | Moreno López, Olatz | |
dc.contributor.author | Kleinbielen, Tamara | |
dc.contributor.author | Ferragut, Joana Francesca | |
dc.contributor.author | Picornell, Antònia | |
dc.contributor.author | Martínez de Pancorbo Gómez, María de los Ángeles | |
dc.date.accessioned | 2022-12-19T17:20:10Z | |
dc.date.available | 2022-12-19T17:20:10Z | |
dc.date.issued | 2022-12 | |
dc.identifier.citation | Scientific Reports 12 : (2022) // Article ID 21734 | es_ES |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10810/58885 | |
dc.description.abstract | Y chromosomal short tandem repeats (Y-STRs) are used in forensic investigations as a useful complementary tool to autosomal markers. The ongoing development of new kits with an increasing number of markers makes it necessary to update populations typed in the Y-STR Haplotype Reference Database to reach at least 23 Y-STRs. A novel Y-STR multiplex panel was developed to offer a cost-efficient alternative to update Y-STR haplotypes from 12 to 23 loci. This panel includes the eleven markers, DYS448, DYS456, DYS458, DYS635, Y-GATA H4, DYS576, DYS481, DYS549, DYS533, DYS570 and DYS643, as well as DYS385a/b for traceability purpose. Developmental validation of this panel was conducted following the recommendations of the Scientific Working Group on DNA Analysis Methods (SWGDAM), showing high sensitivity, tolerance to common inhibitors as well as high species specificity. It was efficient for degraded DNA samples and for detection of male mixtures. When applying it for extending the current data of the Ibiza population, both the discrimination capacity and the haplotype diversity increased from 0.5952 to 0.9048 and from 0.9808 to 0.9977, respectively. Together, the study demonstrates the suitability of this panel in forensic casework. | es_ES |
dc.description.sponsorship | Authors acknowledge funding support from Basque Government under predoctoral fellowship PRE_2021_2_0157 to BNL, PRE_2021_2_0252 to EGR and PRE_2019_2_0005 to TK. Funds were provided by the Basque Government (Grupo Consolidado IT-1271-19). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.title | Development and validation of a new multiplex for upgrading Y-STRs population databases from 12 to 23 markers and its forensic casework application. | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-022-25785-z | es_ES |
dc.identifier.doi | 10.1038/s41598-022-25785-z | |
dc.departamentoes | Zoología y biología celular animal | es_ES |
dc.departamentoeu | Zoologia eta animalia zelulen biologia | es_ES |
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Except where otherwise noted, this item's license is described as © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.