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dc.contributor.authorNieto Garai, Jon Ander
dc.contributor.authorOlazar Intxausti, June
dc.contributor.authorAnso, Itxaso
dc.contributor.authorLorizate Nogales, Maier
dc.contributor.authorTerrones Urio, Oihana ORCID
dc.contributor.authorContreras, F. Xabier
dc.date.accessioned2022-12-20T15:14:01Z
dc.date.available2022-12-20T15:14:01Z
dc.date.issued2022-12-05
dc.identifier.citationInternational Journal of Molecular Sciences 23(23) : (2022) // Article ID 15354es_ES
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10810/58909
dc.description.abstractInterorganelle membrane contact sites (MCS) are areas of close vicinity between the membranes of two organelles that are maintained by protein tethers. Recently, a significant research effort has been made to study MCS, as they are implicated in a wide range of biological functions, such as organelle biogenesis and division, apoptosis, autophagy, and ion and phospholipid homeostasis. Their composition, characteristics, and dynamics can be studied by different techniques, but in recent years super-resolution fluorescence microscopy (SRFM) has emerged as a powerful tool for studying MCS. In this review, we first explore the main characteristics and biological functions of MCS and summarize the different approaches for studying them. Then, we center on SRFM techniques that have been used to study MCS. For each of the approaches, we summarize their working principle, discuss their advantages and limitations, and explore the main discoveries they have uncovered in the field of MCS.es_ES
dc.description.sponsorshipThe APC was funded by grant PID2020-117405GBI00, funded by MCIN/AEI/10.13039/501100011033 and, as appropriate, by “ERDF A way of making Europe” by the “European Union” or by the “European Union NextGenerationEU/PRTR”; by the Basque Government, grant numbers ELKARTEK22/86 and IT1625-22; and by Fundación Ramón Areces, grant number CIVP20S11276.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN/PID2020-117405GBI00es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectsuper-resolution microscopyes_ES
dc.subjectSIMes_ES
dc.subjectTIRFMes_ES
dc.subjectSTEDes_ES
dc.subjectSMLMes_ES
dc.subjectPALMes_ES
dc.subjectSTORMes_ES
dc.subjectorganelleses_ES
dc.subjectmembrane contact siteses_ES
dc.titleSuper-Resolution Microscopy to Study Interorganelle Contact Siteses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2022-12-09T20:23:17Z
dc.rights.holder© 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).es_ES
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/23/15354es_ES
dc.identifier.doi10.3390/ijms232315354
dc.departamentoesBioquímica y biología molecular
dc.departamentoeuBiokimika eta biologia molekularra


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© 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).
Except where otherwise noted, this item's license is described as © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).