Hybrid 3D Printed and Electrospun Multi-Scale Hierarchical Polycaprolactone Scaffolds to Induce Bone Differentiation
dc.contributor.author | González Pujana, Ainhoa | |
dc.contributor.author | Carranza Fernandino, Teresa | |
dc.contributor.author | Santos Vizcaíno, Edorta | |
dc.contributor.author | Igartua Olaechea, Manuela | |
dc.contributor.author | Guerrero Manso, Pedro Manuel | |
dc.contributor.author | Hernández Martín, Rosa María | |
dc.contributor.author | De la Caba Ciriza, María Coro | |
dc.date.accessioned | 2023-01-11T16:26:33Z | |
dc.date.available | 2023-01-11T16:26:33Z | |
dc.date.issued | 2022-12-19 | |
dc.identifier.citation | Pharmaceutics 14(12) : (2022) // Article ID 2843 | es_ES |
dc.identifier.issn | 1999-4923 | |
dc.identifier.uri | http://hdl.handle.net/10810/59235 | |
dc.description.abstract | Complex scaffolds composed of micro- and nano-structures are a key target in tissue engineering and the combination of sequential 3D printing and electrospinning enables the fabrication of these multi-scale structures. In this work, dual 3D printed and electrospun polycaprolactone (PCL) scaffolds with multiple mesh layers were successfully prepared. The scaffold macro- and micro-porosity were assessed by optical and scanning electron microscopy, showing that electrospun fibers formed aligned meshes within the pores of the scaffold. Consequently, the hydrophilicity of the scaffold increased with time, enhancing cell adhesion and growth. Additionally, compression tests in back and forth cycles demonstrated a good shape recovery behavior of the scaffolds. Biological results indicated that hybrid PCL scaffolds are biocompatible and enable a correct cell culture over time. Moreover, MC3T3-E1 preosteoblast culture on the scaffolds promoted the mineralization, increased the alkaline phosphatase (ALP) activity and upregulated the expression of early and late osteogenic markers, namely ALP and osteopontin (OPN), respectively. These results demonstrate that the sequential combination of 3D printing and electrospinning provides a facile method of incorporating fibers within a 3D printed scaffold, becoming a promising approach towards multi-scale hierarchical scaffolds capable of guiding the osteogenic differentiation. | es_ES |
dc.description.sponsorship | Grant PID2021-124294OB-C22 funded by MCI/AEI10.13039/501100011033 and by “ERDF A way of making Europe”. This research was funded by Eusko Jaurlaritza (Grupos Consolidados, No ref: IT448-22 and IT1658-22). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2021-124294OB-C22 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | 3D printing | es_ES |
dc.subject | electrospinning | es_ES |
dc.subject | bone regeneration | es_ES |
dc.title | Hybrid 3D Printed and Electrospun Multi-Scale Hierarchical Polycaprolactone Scaffolds to Induce Bone Differentiation | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2022-12-22T14:35:46Z | |
dc.rights.holder | © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1999-4923/14/12/2843 | es_ES |
dc.identifier.doi | 10.3390/pharmaceutics14122843 | |
dc.departamentoes | Fisiología | |
dc.departamentoes | Ingeniería química y del medio ambiente | |
dc.departamentoes | Farmacia y ciencias de los alimentos | |
dc.departamentoeu | Fisiologia | |
dc.departamentoeu | Ingeniaritza kimikoa eta ingurumenaren ingeniaritza | |
dc.departamentoeu | Farmazia eta elikagaien zientziak |
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Except where otherwise noted, this item's license is described as © 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).