dc.contributor.author | Malo de Molina, Paula | |
dc.contributor.author | Le, Thu Phuong | |
dc.contributor.author | Iturrospe Ibarra, Amaia | |
dc.contributor.author | Gasser, Urs | |
dc.contributor.author | Arbe Méndez, María Aranzazu | |
dc.contributor.author | Colmenero de León, Juan ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Pomposo Alonso, José Adolfo ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.date.accessioned | 2023-01-17T16:33:23Z | |
dc.date.available | 2023-01-17T16:33:23Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | ACS Omega 7(46) : 42163-42169 (2022) | es_ES |
dc.identifier.issn | 2470-1343 | |
dc.identifier.uri | http://hdl.handle.net/10810/59328 | |
dc.description.abstract | The main challenge for the preparation of protein single-chain nanoparticles (SCNPs) is the natural complexity of these macromolecules. Herein, we report the suitable conditions to produce "neat" bovine serum albumin (BSA) single-chain nanoparticles (SCNPs) from partially denatured BSA, which involves denaturation in urea and intramolecular cross-linking below the overlap concentration. We use two disuccinimide ester linkers containing three and six methylene spacer groups: disuccinimidyl glutarate (DSG) and disuccinimidyl suberate (DSS), respectively. Remarkably, the degree of internal cross-linking can be followed simply and efficiently via 1H NMR spectroscopy. The associated structural changes-as probed by small-angle neutron scattering (SANS)-reveal that the denatured protein has a random-like coil conformation, which progressively shrinks with the addition of DSG or DSS, thus allowing for size control of the BSA-SCNPs with radii of gyration down to 5.4 nm. The longer cross-linker exhibits slightly more efficiency in chain compaction with a somewhat stronger size reduction but similar reactivity at a given cross-linker concentration. This reliable method is applicable to a wide range of compact proteins since most proteins have appropriate reactive amino acids and denature in urea. Critically, this work paves the way to the synthesis of "neat", biodegradable protein SCNPs for a range of applications including nanomedicine. | es_ES |
dc.description.sponsorship | The authors acknowledge the financial support received from the IKUR Strategy under the collaboration agreement between the Ikerbasque Foundation and the Materials Physics Center on behalf of the Department of Education of the Basque Government. Financial support by MCIN/AEI/10.13039/501100011033 and “ERDF – A way of making Europe” (grant PID2021-123438NB-I00), Eusko Jaurlaritza – Basque Government (grant IT-1566-22) and the Gipuzkoako Foru Aldundia, Programa Red Gipuzkoana de Ciencia, Tecnología e Innovación (2021-CIEN-000010-01) is gratefully acknowledged. A.I. thanks MICINN for a Personal Técnico de Apoyo contract (PTA2017-14359-I). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2021-123438NB-I00 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | polymer nanoparticles | es_ES |
dc.subject | scattering | es_ES |
dc.subject | dynamics | es_ES |
dc.title | Neat Protein Single-Chain Nanoparticles from Partially Denatured BSA | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 The Authors. Published by
American Chemical Society. Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) | es_ES |
dc.rights.holder | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.relation.publisherversion | https://pubs.acs.org/doi/10.1021/acsomega.2c04805 | es_ES |
dc.identifier.doi | 10.1021/acsomega.2c04805 | |
dc.departamentoes | Polímeros y Materiales Avanzados: Física, Química y Tecnología | es_ES |
dc.departamentoeu | Polimero eta Material Aurreratuak: Fisika, Kimika eta Teknologia | es_ES |