A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors
dc.contributor.author | Rodríguez Abetxuko, Andoni | |
dc.contributor.author | Reifs, Antonio | |
dc.contributor.author | Sánchez de Alcázar Melendo, Daniel | |
dc.contributor.author | Beloqui Elizazu, Ana | |
dc.date.accessioned | 2023-02-10T17:48:24Z | |
dc.date.available | 2023-02-10T17:48:24Z | |
dc.date.issued | 2022-09 | |
dc.identifier.citation | Angewandte Chemie 61(39) : (2022) // Article ID e202206926 | es_ES |
dc.identifier.issn | 1433-7851 | |
dc.identifier.issn | 1521-3773 | |
dc.identifier.uri | http://hdl.handle.net/10810/59758 | |
dc.description.abstract | Herein, we report a multifunctional chemoenzymatic nanoreactor (NanoNOx) for the glucose-controlled regeneration of natural and artificial nicotinamide cofactors. NanoNOx are built of glucose oxidase-polymer hybrids that assemble in the presence of an organometallic catalyst: hemin. The design of the hybrid is optimized to increase the effectiveness and the directional channeling at low substrate concentration. Importantly, NanoNOx can be reutilized without affecting the catalytic properties, can show high stability in the presence of organic solvents, and can effectively oxidize assorted natural and artificial enzyme cofactors. Finally, the hybrid was successfully coupled with NADH-dependent dehydrogenases in one-pot reactions, using a strategy based on the sequential injection of a fuel, namely, glucose. Hence, this study describes the first example of a hybrid chemoenzymatic nanomaterial able to efficiently mimic NOx enzymes in cooperative one-pot cascade reactions. | es_ES |
dc.description.sponsorship | The authors gratefully acknowledge the financial support from the Spanish Research Agency (AEI) for the financial support (PID2019-110239RB-I00 from I+D call and RYC2018-025923-I from RyC program), including FEDER funds; IKERBASQUE-Basque Foundation for Science, BBVA foundation (Leonardo Fellowships, IN[21]_CBB_QUI_0086) and Maria de Maeztu Units of Excellence Programme: Grant Number CEX2020-001038-M. AR-A thanks the Basque Government for his Ph.D. fellowship (PRE_2021_2_0151). Open Access funding is provided by the University of the Basque Country (UPV/EHU). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2019-110239RB-I00 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICIU/RYC2018-025923-I | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/CEX2020-001038-M | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | cascade reactions | es_ES |
dc.subject | chemoenzymatic materials | es_ES |
dc.subject | heterogeneous biocatalysis | es_ES |
dc.subject | nicotinamide cofactor regeneration | es_ES |
dc.title | A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | es_ES |
dc.rights.holder | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/10.1002/anie.202206926 | es_ES |
dc.identifier.doi | 10.1002/anie.202206926 | |
dc.departamentoes | Química aplicada | es_ES |
dc.departamentoeu | Kimika aplikatua | es_ES |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as © 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.