dc.contributor.author | AL Qtaish, Nuseibah | |
dc.contributor.author | Villate Beitia, Ane Ilia | |
dc.contributor.author | Gallego Garrido, Idoia | |
dc.contributor.author | Martínez Navarrete, Gema | |
dc.contributor.author | Soto-Sánchez, Cristina | |
dc.contributor.author | Sainz Ramos, Myriam | |
dc.contributor.author | López Méndez, Tania Belén | |
dc.contributor.author | Paredes, Alejandro Javier | |
dc.contributor.author | Chichón, Francisco Javier | |
dc.contributor.author | Zamarreño, Noelia | |
dc.contributor.author | Fernández, Eduardo | |
dc.contributor.author | Puras Ochoa, Gustavo | |
dc.contributor.author | Pedraz Muñoz, José Luis ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.date.accessioned | 2023-06-23T16:38:54Z | |
dc.date.available | 2023-06-23T16:38:54Z | |
dc.date.issued | 2023-05 | |
dc.identifier.citation | International Journal of Pharmaceutics 639 : (2023) // Article ID 122968 | es_ES |
dc.identifier.issn | 0378-5173 | |
dc.identifier.issn | 1873-3476 | |
dc.identifier.uri | http://hdl.handle.net/10810/61613 | |
dc.description.abstract | Nanodiamonds were combined with niosome, and resulting formulations were named as nanodiasomes, which were evaluated in terms of physicochemical features, cellular internalization, cell viability and transfection efficiency both in in vitro and in in vivo conditions. Such parameters were analyzed at 4 and 25 °C, and at 15 and 30 days after their elaboration. Nanodiasomes showed a particle size of 128 nm that was maintained over time inside the ± 10% of deviation, unless after 30 days of storage at 25 °C. Something similar occurred with the initial zeta potential value, 35.2 mV, being both formulations more stable at 4 °C. The incorporation of nanodiamonds into niosomes resulted in a 4-fold increase of transfection efficiency that was maintained over time at 4 and 25 °C. In vivo studies reported high transgene expression of nanodiasomes after subretinal and intravitreal administration in mice, when injected freshly prepared and after 30 days of storage at 4 °C. | es_ES |
dc.description.sponsorship | This work was supported by the Basque Country Government (Consolidated Groups, IT1448-22 and by CIBER -Consorcio Centro de Investigación Biomédica en Red- CB06/01/1028, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación. Authors wish to thank: ICTS “NANBIOSIS”, specifically the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) for the intellectual and technical assistance. Authors also thank SGIker (UPV/EHU) for technical and human support. Authors acknowledge Rocio Arranz, access to the cryoEM CSIC facility in the context of the CRIOMECORR project (ESFRI-2019-01-CSIC-16). I.V.B. thanks the University of the Basque Country (UPV/EHU) for the granted postdoctoral fellowship (call for the Specialization of Doctor Researcher Personnel of the UPV/EHU, grant reference: ESPDOC19/47). M.S.R. thanks the University of the Basque Country (UPV/EHU) for the granted pre-doctoral fellowship (PIF17/79). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | nanodiamond | es_ES |
dc.subject | niosome | es_ES |
dc.subject | non-viral | es_ES |
dc.subject | gene delivery | es_ES |
dc.subject | stability | es_ES |
dc.subject | retina | es_ES |
dc.title | Long-term biophysical stability of nanodiamonds combined with lipid nanocarriers for non-viral gene delivery to the retina | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0378517323003885 | es_ES |
dc.identifier.doi | 10.1016/j.ijpharm.2023.122968 | |
dc.departamentoes | Fisiología | es_ES |
dc.departamentoeu | Fisiologia | es_ES |