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Molecular Identification of Fungal Species through Multiplex-qPCR to Determine Candidal Vulvovaginitis and Antifungal Susceptibility

dc.contributor.authorArrieta Aguirre, Inés ORCID
dc.contributor.authorMenéndez Manjón Tartière, María del Pilar
dc.contributor.authorCarrano, Giulia ORCID
dc.contributor.authorDíez Villaba, Ander
dc.contributor.authorFernández de Larrinoa Santamaría, Iñigo
dc.contributor.authorMoragues Tosantos, María Dolores ORCID
dc.date.accessioned2024-02-02T16:09:06Z
dc.date.available2024-02-02T16:09:06Z
dc.date.issued2023-11-27
dc.identifier.citationJournal of Fungi 9(12) : (2023) // Article ID 1145es_ES
dc.identifier.issn2309-608X
dc.identifier.urihttp://hdl.handle.net/10810/64595
dc.description.abstractVulvovaginal candidiasis (VVC) is a prevalent condition affecting women worldwide. This study aimed to develop a rapid qPCR assay for the accurate identification of VVC etiological agents and reduced azole susceptibility. One hundred and twenty nine vaginal samples from an outpatient clinic (Bilbao, Spain) were analyzed using culture-based methods and a multiplex qPCR targeting fungal species, which identified Candida albicans as the predominant species (94.2%). Antifungal susceptibility tests revealed reduced azole susceptibility in three (3.48%) isolates. Molecular analysis identified several mutations in genes associated with azole resistance as well as novel mutations in TAC1 and MRR1 genes. In conclusion, we developed a rapid multiplex qPCR assay that detects C. albicans in vulvovaginal specimens and reported new mutations in resistance-related genes that could contribute to azole resistance.es_ES
dc.description.sponsorshipThis research was funded by a Basque Government project, number IT 913-16_Basque University System Research Groups (to M.-D.M.), University of the Basque Country project, GIU21/07_Research Groups of the University of the Basque Country UPV/EHU (to Í.F.-d.-L.), and a Collaborative Research Project among UPV/EHU Groups (to M.-D.M., Í.F.-d.-L. and I.A.) P.M.M received a predoctoral grant from the Department of Education, Universities, and Research of the Basque Government (Programa de Formación de Personal Investigador No Doctor).es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/es/
dc.subjectvulvovaginal candidiasis (VVC)es_ES
dc.subjectqPCRes_ES
dc.subjectCandida specieses_ES
dc.subjectantifungal susceptibilityes_ES
dc.subjectresistance-related mutationses_ES
dc.titleMolecular Identification of Fungal Species through Multiplex-qPCR to Determine Candidal Vulvovaginitis and Antifungal Susceptibilityes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2023-12-22T13:45:28Z
dc.rights.holder© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).es_ES
dc.relation.publisherversionhttps://www.mdpi.com/2309-608X/9/12/1145es_ES
dc.identifier.doi10.3390/jof9121145
dc.departamentoesInmunología, microbiología y parasitología
dc.departamentoesEnfermería I
dc.departamentoesQuímica aplicada
dc.departamentoeuImmunologia, mikrobiologia eta parasitologia
dc.departamentoeuErizaintza I
dc.departamentoeuKimika aplikatua


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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).
Except where otherwise noted, this item's license is described as © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).