Molecular Identification of Fungal Species through Multiplex-qPCR to Determine Candidal Vulvovaginitis and Antifungal Susceptibility
dc.contributor.author | Arrieta Aguirre, Inés | |
dc.contributor.author | Menéndez Manjón Tartière, María del Pilar | |
dc.contributor.author | Carrano, Giulia | |
dc.contributor.author | Díez Villaba, Ander | |
dc.contributor.author | Fernández de Larrinoa Santamaría, Iñigo | |
dc.contributor.author | Moragues Tosantos, María Dolores | |
dc.date.accessioned | 2024-02-02T16:09:06Z | |
dc.date.available | 2024-02-02T16:09:06Z | |
dc.date.issued | 2023-11-27 | |
dc.identifier.citation | Journal of Fungi 9(12) : (2023) // Article ID 1145 | es_ES |
dc.identifier.issn | 2309-608X | |
dc.identifier.uri | http://hdl.handle.net/10810/64595 | |
dc.description.abstract | Vulvovaginal candidiasis (VVC) is a prevalent condition affecting women worldwide. This study aimed to develop a rapid qPCR assay for the accurate identification of VVC etiological agents and reduced azole susceptibility. One hundred and twenty nine vaginal samples from an outpatient clinic (Bilbao, Spain) were analyzed using culture-based methods and a multiplex qPCR targeting fungal species, which identified Candida albicans as the predominant species (94.2%). Antifungal susceptibility tests revealed reduced azole susceptibility in three (3.48%) isolates. Molecular analysis identified several mutations in genes associated with azole resistance as well as novel mutations in TAC1 and MRR1 genes. In conclusion, we developed a rapid multiplex qPCR assay that detects C. albicans in vulvovaginal specimens and reported new mutations in resistance-related genes that could contribute to azole resistance. | es_ES |
dc.description.sponsorship | This research was funded by a Basque Government project, number IT 913-16_Basque University System Research Groups (to M.-D.M.), University of the Basque Country project, GIU21/07_Research Groups of the University of the Basque Country UPV/EHU (to Í.F.-d.-L.), and a Collaborative Research Project among UPV/EHU Groups (to M.-D.M., Í.F.-d.-L. and I.A.) P.M.M received a predoctoral grant from the Department of Education, Universities, and Research of the Basque Government (Programa de Formación de Personal Investigador No Doctor). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/es/ | |
dc.subject | vulvovaginal candidiasis (VVC) | es_ES |
dc.subject | qPCR | es_ES |
dc.subject | Candida species | es_ES |
dc.subject | antifungal susceptibility | es_ES |
dc.subject | resistance-related mutations | es_ES |
dc.title | Molecular Identification of Fungal Species through Multiplex-qPCR to Determine Candidal Vulvovaginitis and Antifungal Susceptibility | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2023-12-22T13:45:28Z | |
dc.rights.holder | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2309-608X/9/12/1145 | es_ES |
dc.identifier.doi | 10.3390/jof9121145 | |
dc.departamentoes | Inmunología, microbiología y parasitología | |
dc.departamentoes | Enfermería I | |
dc.departamentoes | Química aplicada | |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia | |
dc.departamentoeu | Erizaintza I | |
dc.departamentoeu | Kimika aplikatua |
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Except where otherwise noted, this item's license is described as © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).