Catalyst-Free Amino-Yne Click Reaction: An Efficient Way for Immobilizing Amoxicillin onto Polymeric Surfaces
dc.contributor.author | Sánchez Bodón, Julia | |
dc.contributor.author | Díaz Galbarriatu, Maria | |
dc.contributor.author | Sola Llano, Rebeca | |
dc.contributor.author | Ruiz Rubio, Leire | |
dc.contributor.author | Vilas Vilela, José Luis | |
dc.contributor.author | Moreno Benitez, María Isabel | |
dc.date.accessioned | 2024-02-06T16:46:11Z | |
dc.date.available | 2024-02-06T16:46:11Z | |
dc.date.issued | 2024-01-15 | |
dc.identifier.citation | Polymers 16(2) : (2024) // Article ID 246 | es_ES |
dc.identifier.issn | 2073-4360 | |
dc.identifier.uri | http://hdl.handle.net/10810/64694 | |
dc.description.abstract | Surface modifications play a crucial role in enhancing the functionality of biomaterials. Different approaches can be followed in order to achieve the bioconjugation of drugs and biological compounds onto polymer surfaces. In this study, we focused on the immobilization of an amoxicillin antibiotic onto the surface of poly-L-lactic acid (PLLA) using a copper-free amino-yne click reaction. The utilization of this reaction allowed for a selective and efficient bioconjugation of the amoxicillin moiety onto the PLLA surface, avoiding copper-related concerns and ensuring biocompatibility. The process involved sequential steps that included surface activation via alkaline hydrolysis followed by an amidation reaction with ethylendiamine, functionalization with propiolic groups, and subsequent conjugation with amoxicillin via a click chemistry approach. Previous amoxicillin immobilization using tryptophan and fluorescent amino acid conjugation was carried out in order to determine the efficacy of the proposed methodology. Characterization techniques such as X-ray photoelectron spectroscopy (XPS), Attenuated Total Reflection (ATR)–Fourier Transform Infrared (FTIR) spectroscopy, surface imaging, water contact angle determination, and spectroscopic analysis confirmed the successful immobilization of both tryptophan and amoxicillin while maintaining the integrity of the PLLA surface. This tailored modification not only exhibited a novel method for surface functionalization but also opens avenues for developing antimicrobial biomaterials with improved drug-loading capacity. | es_ES |
dc.description.sponsorship | This research was funded by the Basque Government (ELKARTEK program, Department of Development and Infrastructures of the Basque Country, KK-2023-00016); Grupos Consolidados IT1756-22; Ministerio de Ciencia e Innovación (MCIN)/Agencia Estatal de Investigación (AEI) (MCIN/AEI/10.13039/501100011033; and the Basque Government (IT1639-22). Moreover, the authors are grateful for the technical and human support provided by SGIker (UPV/EHU/ERDF, EU). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/es/ | |
dc.subject | click chemistry | es_ES |
dc.subject | amino-yne | es_ES |
dc.subject | bioconjugation | es_ES |
dc.subject | polymer | es_ES |
dc.title | Catalyst-Free Amino-Yne Click Reaction: An Efficient Way for Immobilizing Amoxicillin onto Polymeric Surfaces | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2024-01-26T14:11:07Z | |
dc.rights.holder | © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2073-4360/16/2/246 | es_ES |
dc.identifier.doi | 10.3390/polym16020246 | |
dc.departamentoes | Química física | |
dc.departamentoes | Química Orgánica e Inorgánica | |
dc.departamentoeu | Kimika fisikoa | |
dc.departamentoeu | Kimika Organikoa eta Ez-Organikoa |
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Except where otherwise noted, this item's license is described as © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).