dc.contributor.author | Khmelinskaia, Alena | |
dc.contributor.author | Ibarguren, Maitane ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | de Almeida, Rodrigro F.M. | |
dc.contributor.author | López Jiménez, David ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Paixao, Vanda A. | |
dc.contributor.author | Ahyayauch, Hasna | |
dc.contributor.author | Goñi Urcelay, Félix María ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Escriba, Pablo ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.date.accessioned | 2024-02-08T07:38:27Z | |
dc.date.available | 2024-02-08T07:38:27Z | |
dc.date.issued | 2014-02-03 | |
dc.identifier.citation | Langmuir 30(8) :2117-28 (2014) | es_ES |
dc.identifier.issn | 0743-7463 | |
dc.identifier.other | PMID: 24490728 | |
dc.identifier.uri | http://hdl.handle.net/10810/64791 | |
dc.description.abstract | Recent research regarding 2-hydroxylated fatty acids (2OHFAs) showed clear evidence of their benefits in the treatment of cancer, inflammation, and neurodegenerative disorders such as Alzheimer’s disease. Monolayer compressibility isotherms and isothermal titration calorimetry of 2OHFA (C18−C22) in phosphatidylcholine/phosphatidylethanolamine/sphingomyelin/cholesterol (1:1:1:1 mole ratio), a
mixture that mimics the composition of mammalian plasma membrane, were performed to assess the membrane binding capacity of 2OHFAs and their natural, nonhydroxylated counterparts. The results show that 2OHFAs are surfaceactive substances that bind membranes through exothermic, spontaneous processes. The main effects of 2OHFAs are a decrease in lipid order, with a looser packing of the acyl chains, and a decreased dipole potential, regardless of the 2OHFAs’ relative affinity for the lipid bilayer. The strongest effects are usually observed for 2-hydroxyarachidonic (C20:4) acid, and the weakest one, for 2-hydroxydocosahexaenoic acid (C22:6). In addition, 2OHFAs cause increased hydration, except in gel-phase membranes, which can be explained by the 2OHFA preference for membrane defects. Concerning the membrane dipole potential, the magnitude of the reduction induced by 2OHFAs was particularly marked in the liquid-ordered (lo) phase (cholesterol/sphingomyelin-rich) membranes, those where order reduction was the smallest, suggesting a disruption of cholesterol−sphingolipid interactions that are responsible for the large dipole potential in those membranes. Moreover, 2OHFA effects were larger than for both lo and ld phases separately in model
membranes with liquid disordered (ld)/lo coexistence when both phases were present in significant amounts, possibly because of the facilitating effect of ld/lo domain interfaces. The specific and marked changes induced by 2OHFAs in several membrane properties suggest that the initial interaction with the membrane and subsequent reorganization might constitute an important step in their mechanisms of action. | es_ES |
dc.description.sponsorship | Ministerio de Economía y Competitividad (Torres-Quevedo Research Contracts, PTQ-10-04214 and PTQ-09-02-02113).
Fundación Marathon
Grants to Research Groups of Excellence from Govern de les Illes Balears
Basque Government (IT849-13)
Spanish Ministerio de Ciencia e Innovación (BFU2012-36241, BIO2010-21132)
Portuguese national funds and Fundo Social Europeu through FCT, the Portuguese Foundation for Science and Technology, by “Ciência 2007”and “Investigador FCT 2012” Initiatives (POPH), and by grant PEst-OE/QUI/UI0612/2011-2013.
Fundação Amadeu Dias/Universidade de Lisboa. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Chemical Society | |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.title | Changes in Membrane Organization upon Spontaneous Insertion of 2‑Hydroxylated Unsaturated Fatty Acids in the Lipid Bilayer | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | Copyright © 2014 American Chemical Society | es_ES |
dc.relation.publisherversion | https://pubs.acs.org/doi/10.1021/la403977f | es_ES |
dc.identifier.doi | 10.1021/la403977f | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |