dc.contributor.author | Attia, Noha | |
dc.contributor.author | Santos Vizcaíno, Edorta ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Abdelmouty, Hala | |
dc.contributor.author | Arafa, Samia | |
dc.contributor.author | Zohdy, Nahed | |
dc.contributor.author | Hernández Martín, Rosa María ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Orive Arroyo, Gorka | |
dc.contributor.author | Pedraz Muñoz, José Luis ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.date.accessioned | 2024-02-08T11:26:44Z | |
dc.date.available | 2024-02-08T11:26:44Z | |
dc.date.issued | 2014-04-24 | |
dc.identifier.citation | Journal of Microencapsulation 31(6) : 579-589 (2014) | es_ES |
dc.identifier.issn | 1464-5246 | |
dc.identifier.uri | http://hdl.handle.net/10810/65606 | |
dc.description.abstract | Context: Human bone marrow mesenchymal stem cells (hBM-MSCs) show a great promise for the treatment of a variety of diseases. Despite the previous trials to encapsulate hBM-MSCs in alginate-poly-l-lysine-alginate (APA) systems, the various changes that follow immobilisation have not been ascertained yet. Objective: Determine the various consequences derived from entrapment on cell behaviour, putting special emphasis on the ultrastructure. Methods: hBM-MSCs were immobilised in APA microcapsules to further characterise their viability, metabolic activity, proliferation, VEGF-secretability, and morphology. Results: The VEGF produced by monolayer hBM-MSCs increased significantly 1 d post-encapsulation, and was maintained for at least 4 weeks. TEM imaging of cells revealed well preserved ultrastructure indicating protein synthesis and high metabolic activity. Conclusion: Although APA microencapsulation did not support 100% of fully viable hBM-MSCs for long-term cultures, it was conceived to enhance both VEGF secretion and metabolic activity while not losing their stemness characteristics. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Taylor & Francis | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | alginate | es_ES |
dc.subject | bone marrow-derived mesenchymal stem cells | |
dc.subject | cell microencapsulation | |
dc.subject | ultrastructure | |
dc.subject | VEGF secretion | |
dc.title | Behavior And Ultrastructure Of Human Bone Marrow-Derived Mesenchymal Stem Cells Immobilized In Alginate-Poly-L-Lysine-Alginate Microcapsules | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | ©2014 Informa | * |
dc.relation.publisherversion | https://www.tandfonline.com/doi/full/10.3109/02652048.2014.898706 | |
dc.identifier.doi | 10.3109/02652048.2014.898706 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | es_ES |
dc.departamentoeu | Farmazia eta elikagaien zientziak | es_ES |