dc.contributor.author | Arana Urbieta, Lide | |
dc.contributor.author | Gangoiti Muñecas, Patricia | |
dc.contributor.author | Ouro Villasante, Alberto | |
dc.contributor.author | Rivera Líbano, Io Guané | |
dc.contributor.author | Ordóñez Zaragoza, Marta | |
dc.contributor.author | Trueba Conde, Miguel Ángel | |
dc.contributor.author | Lankalapalli, Ravi S. | |
dc.contributor.author | Bittman, Robert | |
dc.contributor.author | Gómez Muñoz, Antonio | |
dc.date.accessioned | 2024-02-09T16:35:09Z | |
dc.date.available | 2024-02-09T16:35:09Z | |
dc.date.issued | 2011-12-06 | |
dc.identifier.citation | Experimental Cell Research 318(4) : 350-360 (2012) | es_ES |
dc.identifier.issn | 0014-4827 | |
dc.identifier.issn | 1090-2422 | |
dc.identifier.uri | http://hdl.handle.net/10810/65968 | |
dc.description.abstract | We previously demonstrated that ceramide 1-phosphate (C1P) is mitogenic for fibroblasts and macrophages. However, the mechanisms involved in this action were only partially described. Here, we demonstrate that C1P stimulates reactive oxygen species (ROS) formation in primary bone marrow-derived macrophages, and that ROS are required for the mitogenic effect of C1P. ROS production was dependent upon prior activation of NADPH oxidase by C1P, which was determined by measuring phosphorylation of the p40phox subunit and translocation of p47phox from the cytosol to the plasma membrane. In addition, C1P activated cytosolic calcium-dependent phospholipase A2 and protein kinase C-, and NADPH oxidase activation was blocked by selective inhibitors of these enzymes. These inhibitors, and inhibitors of ROS production, blocked the mitogenic effect of C1P. By using BHNB-C1P (a photolabile caged-C1P analog), we demonstrate that all of these C1P actions are caused by intracellular C1P. It can be concluded that the enzyme responsible for C1P-stimulated ROS generation in bone marrow-derived macrophages is NADPH oxidase, and that this enzyme is downstream of PKC- and cPLA2- in this pathway. | es_ES |
dc.description.sponsorship | This work was supported by grants BFU2009-13314/BFI from
Ministerio de Ciencia e Innovación (MICINN) (Madrid, Spain),
IT-353-10 from Departamento de Educación, Universidades e
Investigación del Gobierno Vasco (GV/EJ), SA-2010/00013 from
Departamento de Industria, Comercio y Turismo del Gobierno
Vasco (Basque Government, GV/EJ) to AGM, and grant HL083187
from the National Institutes of Health to RB. LA and AO are the recipients of fellowships from the Basque Government. | |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/FU2009-13314/BFI | |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | ceramides | es_ES |
dc.subject | ceramide 1-phosphate | es_ES |
dc.subject | NADPH oxidase | es_ES |
dc.subject | ROS | es_ES |
dc.subject | proliferation | es_ES |
dc.subject | sphingolipids | es_ES |
dc.title | Generation of Reactive Oxygen Species (ROS) is a key factor for stimulation of macrophage proliferation by ceramide 1-phosphate | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2011 Elsevier under CC BY-NC-ND license | * |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S001448271100470 | |
dc.identifier.doi | /10.1016/j.yexcr.2011.11.013 | |