Serological Antibodies against Kidney, Liver, and Spleen Membrane Antigens as Potential Biomarkers in Patients with Immune Disorders
dc.contributor.author | Hernández Suárez, Leidi | |
dc.contributor.author | Díez Martín, Eguzkiñe | |
dc.contributor.author | Egiguren Ortiz, June | |
dc.contributor.author | Fernández, Roberto | |
dc.contributor.author | Etxebarria Gallego, Aitor | |
dc.contributor.author | Astigarraga Arribas, Egoitz | |
dc.contributor.author | Miguélez Palomo, Cristina | |
dc.contributor.author | Ramírez Garcia, Andoni | |
dc.contributor.author | Barreda Gómez, Gabriel | |
dc.date.accessioned | 2024-04-10T15:16:33Z | |
dc.date.available | 2024-04-10T15:16:33Z | |
dc.date.issued | 2024-02-07 | |
dc.identifier.citation | International Journal of Molecular Sciences 25(4) : (2024) // Article ID 2025 | es_ES |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/10810/66602 | |
dc.description.abstract | Immune disorders arise from complex genetic and environmental factors, which lead to dysregulation at the cellular and inflammatory levels and cause tissue damage. Recent research highlights the crucial role of reactive antibodies in autoimmune diseases and graft rejection, but their complex determination poses challenges for clinical use. Therefore, our study aimed to ascertain whether the presence of reactive antibodies against membrane antigens in tissues from both animal models and humans could serve as biomarkers in patients with autoimmune disorders. To address this issue, we examined the binding profile of serological antibodies against a diverse panel of cell membranes from the spleen, liver, and kidney tissues of monkeys, rats, and humans. After developing the cell membrane microarrays, human sera were immunologically assayed. The study was first conducted on sera from two groups, healthy subjects and patients with inflammatory and autoimmune disorders, and then optimized for kidney transplant patient sera. A significant increase in antibody reactivity against specific monkey kidney and spleen membranes was observed in the serum of patients with lupus nephritis, while kidney transplant patients showed a significant enhancement against human tissues and human embryonic kidney 293 cells. These results show the potential importance for clinical and basic research purposes of studying the presence of specific IgG against membrane antigens in patients’ serum as potential biomarkers of immune disorders. However, it is important to note that these results need to be verified in further studies with a larger sample size to confirm their relevance. | es_ES |
dc.description.sponsorship | This research was funded by the Spanish Ministry of Science and Innovation Center for Technological and Industrial Development (CDTI) (exp. PTQ2019-010650), Basque Government Department of Economic Development, Sustainability and Environment Bikaintek program, grant number 004-B2/2022, 005-B2/2021 and 006-B2/2021, and University of the Basque Country, UPV/EHU, grant number PIFIND21/02 and PIFIND21/04. In addition, this research was funded by Basque Government: Grants for Consolidated Research Groups (IT1657-22). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/es/ | |
dc.subject | inflammation | es_ES |
dc.subject | autoimmune disorders | es_ES |
dc.subject | reactive antibodies | es_ES |
dc.subject | microarray | es_ES |
dc.title | Serological Antibodies against Kidney, Liver, and Spleen Membrane Antigens as Potential Biomarkers in Patients with Immune Disorders | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2024-02-23T15:03:58Z | |
dc.rights.holder | © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/25/4/2025 | es_ES |
dc.identifier.doi | 10.3390/ijms25042025 | |
dc.departamentoes | Inmunología, microbiología y parasitología | |
dc.departamentoes | Farmacología | |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia | |
dc.departamentoeu | Farmakologia |
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Except where otherwise noted, this item's license is described as © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).