Isolation and Differentiation of Neurons and Glial Cells from Olfactory Epithelium in Living Subjects
dc.contributor.author | Unzueta Larrinaga, Paula | |
dc.contributor.author | Barrena Barbadillo, Rocío | |
dc.contributor.author | Ibarra Lecue, Inés | |
dc.contributor.author | Horrillo Furundarena, Igor | |
dc.contributor.author | Villate Uribe, Aitor | |
dc.contributor.author | Recio Barbero, María | |
dc.contributor.author | Meana Martínez, José Javier | |
dc.contributor.author | Díez Alarcia, Rebeca | |
dc.contributor.author | Mentxaka Solozabal, Oihane | |
dc.contributor.author | Segarra Echevarria, Rafael | |
dc.contributor.author | Etxebarria Loizate, Nestor | |
dc.contributor.author | Callado Hernando, Luis Felipe | |
dc.contributor.author | Urigüen Echeverría, Leyre | |
dc.date.accessioned | 2024-05-07T16:45:37Z | |
dc.date.available | 2024-05-07T16:45:37Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Molecular Neurobiology 60 : 4472-4487 (2023) | es_ES |
dc.identifier.issn | 1559-1182 | |
dc.identifier.issn | 0893-7648 | |
dc.identifier.uri | http://hdl.handle.net/10810/67664 | |
dc.description.abstract | The study of psychiatric and neurological diseases requires the substrate in which the disorders occur, that is, the nervous tissue. Currently, several types of human bio-specimens are being used for research, including postmortem brains, cerebrospinal fluid, induced pluripotent stem (iPS) cells, and induced neuronal (iN) cells. However, these samples are far from providing a useful predictive, diagnostic, or prognostic biomarker. The olfactory epithelium is a region close to the brain that has received increased interest as a research tool for the study of brain mechanisms in complex neuropsychiatric and neurological diseases. The olfactory sensory neurons are replaced by neurogenesis throughout adult life from stem cells on the basement membrane. These stem cells are multipotent and can be propagated in neurospheres, proliferated in vitro and differentiated into multiple cell types including neurons and glia. For all these reasons, olfactory epithelium provides a unique resource for investigating neuronal molecular markers of neuropsychiatric and neurological diseases. Here, we describe the isolation and culture of human differentiated neurons and glial cells from olfactory epithelium of living subjects by an easy and non-invasive exfoliation method that may serve as a useful tool for the research in brain diseases. | es_ES |
dc.description.sponsorship | Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This study was funded by the Spanish Ministry of Science and Innovation (PID2019-106404RB-I00), Spanish Ministry of Health (PNSD 2019I021), and Basque Government (2019111082, IT1211/19 and IT1512/22). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | olfactory epithelium | es_ES |
dc.subject | neurospheres | es_ES |
dc.subject | neurons | es_ES |
dc.subject | glia | es_ES |
dc.subject | neuropsychiatric diseases | es_ES |
dc.subject | PSA-NCAM | es_ES |
dc.title | Isolation and Differentiation of Neurons and Glial Cells from Olfactory Epithelium in Living Subjects | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://link.springer.com/article/10.1007/s12035-023-03363-2 | es_ES |
dc.identifier.doi | 10.1007/s12035-023-03363-2 | |
dc.departamentoes | Farmacología | es_ES |
dc.departamentoes | Neurociencias | es_ES |
dc.departamentoes | Química analítica | es_ES |
dc.departamentoeu | Farmakologia | es_ES |
dc.departamentoeu | Kimika analitikoa | es_ES |
dc.departamentoeu | Neurozientziak | es_ES |
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Except where otherwise noted, this item's license is described as © The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.