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dc.contributor.authorCriado González, Miryam
dc.contributor.authorBondi, Luca
dc.contributor.authorMarzuoli, Camilla
dc.contributor.authorGutiérrez Fernández, Edgar
dc.contributor.authorTullii, Gabriele
dc.contributor.authorRonchi, Carlotta
dc.contributor.authorGabirondo Amenabar, Elena
dc.contributor.authorSardon Muguruza, Haritz
dc.contributor.authorRapino, Stefania
dc.contributor.authorMalferrari, Marco
dc.contributor.authorCramer, Tobias
dc.contributor.authorAntognazza, Rosa Maria
dc.contributor.authorMecerreyes Molero, David
dc.date.accessioned2024-05-23T17:07:37Z
dc.date.available2024-05-23T17:07:37Z
dc.date.issued2023-07
dc.identifier.citationACS Applied Materials & Interfaces 15(30) : 35973-35985 (2023)es_ES
dc.identifier.issn1944-8244
dc.identifier.issn1944-8252
dc.identifier.urihttp://hdl.handle.net/10810/68134
dc.description.abstractThe design of soft and nanometer-scale photoelectrodes able to stimulate and promote the intracellular concentration of reactive oxygen species (ROS) is searched for redox medicine applications. In this work, we show semiconducting polymer porous thin films with an enhanced photoelectrochemical generation of ROS in human umbilical vein endothelial cells (HUVECs). To achieve that aim, we synthesized graft copolymers, made of poly(3-hexylthiophene) (P3HT) and degradable poly(lactic acid) (PLA) segments, P3HT-g-PLA. In a second step, the hydrolysis of sacrificial PLA leads to nanometer-scale porous P3HT thin films. The pore sizes in the nm regime (220–1200 nm) were controlled by the copolymer composition and the structural arrangement of the copolymers during the film formation, as determined by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The porous P3HT thin films showed enhanced photofaradaic behavior, generating a higher concentration of ROS in comparison to non-porous P3HT films, as determined by scanning electrochemical microscopy (SECM) measurements. The exogenous ROS production was able to modulate the intracellular ROS concentration in HUVECs at non-toxic levels, thus affecting the physiological functions of cells. Results presented in this work provide an important step forward in the development of new tools for precise, on-demand, and non-invasive modulation of intracellular ROS species and may be potentially extended to many other physiological or pathological cell models.es_ES
dc.language.isoenges_ES
dc.publisherACSes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.titleSemiconducting polymer nanoporous thin films as a tool to regulate intracellular ROS balance in endothelial cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holder© 2023 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY 4.0.es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://pubs.acs.org/doi/full/10.1021/acsami.3c06633es_ES
dc.identifier.doi10.1021/acsami.3c06633
dc.departamentoesPolímeros y Materiales Avanzados: Física, Química y Tecnologíaes_ES
dc.departamentoeuPolimero eta Material Aurreratuak: Fisika, Kimika eta Teknologiaes_ES


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© 2023 The Authors. Published by American Chemical Society. This publication is licensed under
CC-BY 4.0.
Except where otherwise noted, this item's license is described as © 2023 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY 4.0.