dc.contributor.author | Apellaniz Unzalu, Beatriz | |
dc.contributor.author | García Sáez, Ana J. | |
dc.contributor.author | Huarte Arrayago, Nerea | |
dc.contributor.author | Kunert, Renate | |
dc.contributor.author | Vorauer-Uhl, Karola | |
dc.contributor.author | Katinger, Hermann | |
dc.contributor.author | Schwille, Petra | |
dc.contributor.author | Nieva Escandón, José Luis | |
dc.date.accessioned | 2024-07-11T07:03:30Z | |
dc.date.available | 2024-07-11T07:03:30Z | |
dc.date.issued | 2010-03-17 | |
dc.identifier.citation | Febs Letters 584(8) : 1591-1596 (2010) | es_ES |
dc.identifier.issn | 0014-5793 | |
dc.identifier.uri | http://hdl.handle.net/10810/68862 | |
dc.description.abstract | The broadly neutralizing anti-HIV-1 2F5 monoclonal antibody recognizes a gp41 epitope proximal to the viral membrane. Potential phospholipid autoreactivity at cell surfaces has raised concerns about the use of this antibody for development of vaccines or immunotherapy. In this study, confocal microscopy of giant unilamellar vesicles (GUVs) was used to assess 2F5 reactivity with phospholipids assembled into bilayers with surface charge and curvature stress approximating those of the eukaryotic plasma membranes. Antibody partitioning into lipid bilayers required the specific recognition of membrane-inserted epitope, indicating that 2F5 was unable to directly react with GUV phospholipids, even under fluid phase segregation conditions. Our results thus support the feasibility of raising 2F5-like neutralizing responses through vaccination, and the medical safety of mAb infusions. | es_ES |
dc.description.sponsorship | This study was supported by Spanish MICINN (BIO2008-00772) and University of the Basque Country (GIU 06/42 and DIPE08/12) (to J.L.N.), by the Max-Planck Society (A.J.G.S.) and by Polymun Scientific GmbH, Vienna, Austria. BA was recipient of a pre-doctoral MEC-FPU fellowship. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/BIO2008-00772 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | cholesterol | es_ES |
dc.subject | 19-dioctadecyl-3,3,39,39-tetramethylindodicarbocyanine perchlorate | es_ES |
dc.subject | 1'-dioctadecyl-3,3'-oxacarbocyanine perchlorate | es_ES |
dc.subject | dioleoyl-phosphatidylcholine | es_ES |
dc.subject | giant unilamellar vesicle | es_ES |
dc.subject | multilamellar vesicle | es_ES |
dc.subject | membrane-proximal external region | es_ES |
dc.subject | 1-palmitoyl-2-oleoylphosphatidylcholine | es_ES |
dc.subject | supported-lipid bilayers | es_ES |
dc.subject | sphingomyelin | es_ES |
dc.title | Confocal microscopy of giant vesicles supports the absence of HIV-1 neutralizing 2F5 antibody reactivity to plasma membrane phospholipids | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.febslet.2010.03.021 | es_ES |
dc.identifier.doi | 10.1016/j.febslet.2010.03.021 | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |