Metabolomic Profiling of Fatty Acid and Amino AcidMetabolism in Youth With Obesity and Type 2 Diabetes

Mihalik, Stephanie J.; Michaliszyn, Sara F.; De las Heras Montero, Javier Adolfo; Bacha, Fida; Chace, Donald H.; Lee, SoJung; Chace, Donald H.; DeJesus, Victor R.; Vockley, Jerry; Arslanian, Silva A.

dc.contributor.author	Mihalik, Stephanie J.
dc.contributor.author	Michaliszyn, Sara F.
dc.contributor.author	De las Heras Montero, Javier Adolfo
dc.contributor.author	Bacha, Fida
dc.contributor.author	Chace, Donald H.
dc.contributor.author	Lee, SoJung
dc.contributor.author	Chace, Donald H.
dc.contributor.author	DeJesus, Victor R.
dc.contributor.author	Vockley, Jerry
dc.contributor.author	Arslanian, Silva A.
dc.date.accessioned	2024-10-15T12:29:30Z
dc.date.available	2024-10-15T12:29:30Z
dc.date.issued	2012
dc.identifier.citation	Diabetes Care 35(3) : 605-611 (2012)	es_ES
dc.identifier.issn	0149-5992
dc.identifier.issn	1935-5548
dc.identifier.uri	http://hdl.handle.net/10810/69941
dc.description.abstract	OBJECTIVE We compared acylcarnitine (AcylCN) species, common amino acid and fat oxidation (FOX) byproducts, and plasma amino acids in normal weight (NW; n = 39), obese (OB; n = 64), and type 2 diabetic (n = 17) adolescents. RESEARCH DESIGN AND METHODS Fasting plasma was analyzed by tandem mass spectrometry, body composition by dual energy X-ray absorptiometry and computed tomography, and total-body lipolysis and substrate oxidation by [2H5]glycerol and indirect calorimetry, respectively. In vivo insulin sensitivity (IS) was assessed with a 3-h hyperinsulinemic-euglycemic clamp. RESULTS Long-chain AcylCNs (C18:2-CN to C14:0-CN) were similar among the three groups. Medium- to short-chain AcylCNs (except C8 and C10) were significantly lower in type 2 diabetes compared with NW, and when compared with OB, C2-, C6-, and C10-CN were lower. Amino acid concentrations were lower in type 2 diabetes compared with NW. Fasting lipolysis and FOX were higher in OB and type 2 diabetes compared with NW, and the negative association of FOX to C10:1 disappeared after controlling for adiposity, Tanner stage, and sex. IS was lower in OB and type 2 diabetes with positive associations between IS and arginine, histidine, and serine after adjusting for adiposity, Tanner stage, and sex. CONCLUSIONS These metabolomics results, together with the increased rates of in vivo FOX, are not supportive of defective fatty acid or amino acid metabolism in obesity and type 2 diabetes in youth. Such observations are consistent with early adaptive metabolic plasticity in youth, which over time—with continued obesity and aging—may become dysfunctional, as observed in adults.	es_ES
dc.description.sponsorship	his study was supported by grants R01 HD-27503 (S.A.A.) and K24 HD-01357 (S.A.A.), the Richard L. Day Endowed Chair (S.A.A.), the Department of Defense (S.A.A., S.J.L., and F.B.), American Diabetes Association Grant 7-08-JF-27 (S.J.L.), the Thrasher Research Fund (F.B.), and National Institutes of Health grants ROI DK-78775 (J.V.) and UL1 RR024153 CTSA (previously M01_RR-00084).	es_ES
dc.language.iso	eng	es_ES
dc.publisher	American Diabetes Association	es_ES
dc.rights	info:eu-repo/semantics/openAccess	es_ES
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.title	Metabolomic Profiling of Fatty Acid and Amino AcidMetabolism in Youth With Obesity and Type 2 Diabetes	es_ES
dc.type	info:eu-repo/semantics/article	es_ES
dc.rights.holder	This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed.	es_ES
dc.relation.publisherversion	https://diabetesjournals.org/care/article/35/3/605/28755/Metabolomic-Profiling-of-Fatty-Acid-and-Amino-Acid	es_ES
dc.identifier.doi	10.2337/DC11-1577
dc.departamentoes	Pediatría	es_ES
dc.departamentoeu	Pediatria	es_ES

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This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed.

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