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dc.contributor.authorEcheveste Juárez, Oier
dc.contributor.authorHerrero García, Erika
dc.contributor.authorCortese, Marc S.
dc.contributor.authorGarcía Aguirrezabala, Aitor
dc.contributor.authorOiartzabal Arano, Elixabet
dc.contributor.authorDe los Ríos, Vivian
dc.contributor.authorUgalde Martínez, Unai
dc.contributor.authorEspeso, Eduardo A.
dc.date.accessioned2013-01-31T19:01:26Z
dc.date.available2013-01-31T19:01:26Z
dc.date.issued2012-07-05
dc.identifier.citationPLoS ONE 7(7) : (2012) // e40292.es
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10810/9307
dc.description18 p.es
dc.description.abstractAspergillus nidulans asexual differentiation is induced by Upstream Developmental Activators (UDAs) that include the bZIP-type Transcription Factor (TF) FlbB. A 2D-PAGE/MS-MS-coupled screen for proteins differentially expressed in the presence and absence of FlbB identified 18 candidates. Most candidates belong to GO term classes involved in osmotic and/or oxidative stress response. Among these, we focused on GmcA, a putative glucose-methanol-choline oxidoreductase which is upregulated in a ΔflbB background. GmcA is not required for growth since no differences were detected in the radial extension upon deletion of gmcA. However, its activity is required to induce conidiation under specific culture conditions. A ΔgmcA strain conidiates profusely under acid conditions but displays a characteristic fluffy aconidial phenotype in alkaline medium. The absence of asexual development in a ΔgmcA strain can be suppressed, on one hand, using high concentrations of non-fermentable carbon sources like glycerol, and on the other hand, when the cMyb-type UDA TF flbD is overexpressed. Overall, the results obtained in this work support a role for GmcA at early stages of conidiophore initiation.es
dc.description.sponsorshipThis work has been supported by the Basque Government (SA-2010/00105 and IT393-10) and Ministerio de Ciencia e Innovación (BFU2010-17528) to U.U. and to E.A.E. through grant BFU2009-08701. O.E was a contract researcher of The University of The Basque Country and is now associated to grant BFU2010-17528. E.H.-G. and A.G. held a predoctoral fellowship from the Basque Government, and M.S.C. was a contract researcher of the Ikerbasque program of the Basque Government (www.ikerbasque.net). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relationinfo:eu-repo/grantAgreement/MICINN/BFU2010-17528
dc.relationinfo:eu-repo/grantAgreement/MICINN/BFU2009-08701
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjecttranscription factor FlbBes
dc.subjectoxidoreductasees
dc.subjectSwiss-model workspacees
dc.subjectaryl-alcohol oxidasees
dc.subjectfunctional-characterizationes
dc.subjectproteines
dc.subjectgenees
dc.subjectdatabasees
dc.subjectfungies
dc.subjectlightes
dc.titleGmcA Is a Putative Glucose-Methanol-Choline Oxidoreductase Required for the Induction of Asexual Development in Aspergillus nidulanses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2012 Etxebeste et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.es
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0040292es
dc.identifier.doi10.1371/journal.pone.0040292
dc.departamentoesQuímica aplicadaes_ES
dc.departamentoeuKimika aplikatuaes_ES
dc.subject.categoriaAGRICULTURAL AND BIOLOGICAL SCIENCES
dc.subject.categoriaMEDICINE
dc.subject.categoriaBIOCHEMISTRY AND MOLECULAR BIOLOGY


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