BackBrowsing Artículos by Author "Vicente Pascual, Mónica"
Now showing items 1-7 of 7
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Gene delivery in the cornea: in vitro and ex vivo evaluation of solid lipid nanoparticle-based vectors
; ; Solinís Aspiazu, María Ángeles
; ; ; ; ; ; Del Pozo Rodríguez, Ana ; (Future Medicine, 2018-08-01)
Inflammation is a process that underlies sight-threatening ocular surface diseases, and gene supplementation with the plasmid that encodes for interleukin-10 (p- IL10) will allow the sustained de novo synthesis of the ... -
MMP-9 Downregulation with Lipid Nanoparticles for Inhibiting Corneal Neovascularization by Gene Silencing
Torrecilla Alzola, Josune; Gómez Aguado, Itziar; Vicente Pascual, Mónica; Del Pozo Rodríguez, Ana ; Solinís Aspiazu, María Ángeles ; (MDPI, 2019-04-18)
Gene silencing targeting proangiogenic factors have been shown to be a useful strategy in the treatment of corneal neovascularization (CNV). Among interference RNA (RNAi) molecules, short-hairpin RNA (shRNA) is a plasmid-coded ... -
mRNA-Based Nanomedicinal Products to Address Corneal Inflammation by Interleukin-10 Supplementation
Gómez Aguado, Itziar; Rodríguez Castejón, Julen ; Beraza Millor, Marina ; Vicente Pascual, Mónica; ; Garelli, Sara; Battaglia, Luigi; Del Pozo Rodríguez, Ana ; Solinís Aspiazu, María Ángeles (MDPI, 2021-09-15)
The anti-inflammatory cytokine Interleukin-10 (IL-10) is considered an efficient treatment for corneal inflammation, in spite of its short half-life and poor eye bioavailability. In the present work, mRNA-based nanomedicinal ... -
Nanomedicines to Deliver mRNA: State of the Art and Future Perspectives
Gómez Aguado, Itziar; Rodríguez Castejón, Julen ; Vicente Pascual, Mónica; ; Solinís Aspiazu, María Ángeles ; Del Pozo Rodríguez, Ana (MDPI, 2020-02-20)
The use of messenger RNA (mRNA) in gene therapy is increasing in recent years, due to its unique features compared to plasmid DNA: Transient expression, no need to enter into the nucleus and no risk of insertional mutagenesis. ... -
Nucleic Acid Delivery by Solid Lipid Nanoparticles Containing Switchable Lipids: Plasmid DNA vs. Messenger RNA
Gómez Aguado, Itziar; Rodríguez Castejón, Julen ; Vicente Pascual, Mónica; ; Del Pozo Rodríguez, Ana ; Solinís Aspiazu, María Ángeles (MDPI, 2020-12-18)
The development of safe and effective nucleic acid delivery systems remains a challenge, with solid lipid nanoparticle (SLN)-based vectors as one of the most studied systems. In this work, different SLNs were developed, ... -
Topical Administration of SLN-Based Gene Therapy for the Treatment of Corneal Inflammation by De Novo IL-10 Production
Vicente Pascual, Mónica; Gómez Aguado, Itziar; Rodríguez Castejón, Julen ; ; Muntoni, Elisabetta; Battaglia, Luigi; Del Pozo Rodríguez, Ana ; Solinís Aspiazu, María Ángeles (MDPI, 2020-06-23)
One of the main challenges in gene therapy is the issue of delivery, and it is especially relevant for the success of gene therapy in the cornea. In the present work, eye drops containing biocompatible non-viral vectors ... -
α-Galactosidase A Augmentation by Non-Viral Gene Therapy: Evaluation in Fabry Disease Mice
Rodríguez Castejón, Julen; Alarcia Lacalle, Ana; Gómez Aguado, Itziar ; Vicente Pascual, Mónica; Solinís Aspiazu, María Ángeles ; Del Pozo Rodríguez, Ana ; (MDPI, 2021-05-21)
Fabry disease (FD) is a monogenic X-linked lysosomal storage disorder caused by a deficiency in the lysosomal enzyme α-Galactosidase A (α-Gal A). It is a good candidate to be treated with gene therapy, in which moderately ...