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Transcription Factor Binding Site Enrichment Analysis In Co-Expression Modules In Celiac Disease
(MDPI, 2018-05-10)
The aim of this study was to construct celiac co-expression patterns at a whole genome level and to identify transcription factors (TFs) that could drive the gliadin-related changes in coordination of gene expression ...
A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation
(Nature Publishing Group, 2019-12)
N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in the regulation of several biological processes, including the immune response, and different approaches ...
Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments
(Cell Press, 2020-08-18)
The contribution of membrane interfacial interactions to recognition of membrane-embedded antigens by antibodies is currently unclear. This report demonstrates the optimization of this type of antibodies via chemical ...
Super-Resolution Microscopy Using a Bioorthogonal-Based Cholesterol Probe Provides Unprecedented Capabilities for Imaging Nanoscale Lipid Heterogeneity in Living Cells
(Wiley, 2021-09)
Despite more than 20 years of work since the lipid raft concept was proposed, the existence of these nanostructures remains highly controversial due to the lack of noninvasive methods to investigate their native nanorganization ...
Using a Simple Cellular Assay to Map NES Motifs in Cancer-Related Proteins, Gain Insight into CRM1-Mediated NES Export, and Search for NES-Harboring Micropeptides
(MDPI, 2020-09-01)
The nuclear export receptor CRM1 (XPO1) recognizes and binds specific sequence motifs termed nuclear export signals (NESs) in cargo proteins. About 200 NES motifs have been identified, but over a thousand human proteins ...
Engineering pan–HIV-1 neutralization potency through multispecific antibody avidity
(National Academy of Sciences of the United States of America, 2022-01-25)
Deep mining of B cell repertoires of HIV-1-infected individuals has resulted in the isolation of dozens of HIV-1 broadly neutralizing antibodies (bNAbs). Yet, it remains uncertain whether any such bNAbs alone are sufficiently ...
Fluorescence Microscopy of the HIV-1 Envelope
(MDPI, 2020-03-21)
Human immunodeficiency virus (HIV) infection constitutes a major health and social issue worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane. This process is mediated by the viral Env ...
Structural insights into the ability of nucleoplasmin to assemble and chaperone histone octamers for DNA deposition
(Nature Publishing Group, 2019-07-01)
Nucleoplasmin (NP) is a pentameric histone chaperone that regulates the condensation state of chromatin in different cellular processes. We focus here on the interaction of NP with the histone octamer, showing that NP could ...
Lipid-Assisted Polymerization of Nucleotides
(MDPI, 2019-11-05)
In addition to being one of the proponents of the "Lipid World hypothesis", David Deamer, together with other colleagues, pioneered studies involving formation of RNA-like oligomers from their 'non-activated', prebiotically ...
Solvent-Based Elimination of Organic Matter from Marine-Collected Plastics
(MDPI, 2021-07-20)
The physical-chemical characterization of plastic litter from the marine environment requires the prior removal of the biofouling attached to their surface without causing any degradation in the polymer. The absence of a ...