Microtechnologies for Cell Microenvironment Control and Monitoring
dc.contributor.author | Azuaje Hualde, Enrique | |
dc.contributor.author | García Hernando, Maite | |
dc.contributor.author | Etxebarria Elezgarai, Jaione | |
dc.contributor.author | Martínez de Pancorbo Gómez, María de los Angeles | |
dc.contributor.author | Benito López, Fernando | |
dc.contributor.author | Basabe Desmonts, Lourdes | |
dc.date.accessioned | 2018-05-28T13:36:46Z | |
dc.date.available | 2018-05-28T13:36:46Z | |
dc.date.issued | 2017-06 | |
dc.identifier.citation | Micromachines 8(6) : (2017) // Article ID 866 | es_ES |
dc.identifier.issn | 2072-666X | |
dc.identifier.uri | http://hdl.handle.net/10810/27141 | |
dc.description.abstract | A great breadth of questions remains in cellular biology. Some questions cannot be answered using traditional analytical techniques and so demand the development of new tools for research. In the near future, the development of highly integrated microfluidic analytical platforms will enable the acquisition of unknown biological data. These microfluidic systems must allow cell culture under controlled microenvironment and high throughput analysis. For this purpose, the integration of a variable number of newly developed micro- and nano-technologies, which enable control of topography and surface chemistry, soluble factors, mechanical forces and cell-cell contacts, as well as technology for monitoring cell phenotype and genotype with high spatial and temporal resolution will be necessary. These multifunctional devices must be accompanied by appropriate data analysis and management of the expected large datasets generated. The knowledge gained with these platforms has the potential to improve predictive models of the behavior of cells, impacting directly in better therapies for disease treatment. In this review, we give an overview of the microtechnology toolbox available for the design of high throughput microfluidic platforms for cell analysis. We discuss current microtechnologies for cell microenvironment control, different methodologies to create large arrays of cellular systems and finally techniques for monitoring cells in microfluidic devices. | es_ES |
dc.description.sponsorship | E.A.-H. acknowledges funding from the Basque Government, Department of Education, for predoctoral fellowship 2016. M.G.-H. acknowledges funding from the University of the Basque Country UPV/EHU, PIF16/204 predoctoral fellowship "call for recruitment of research personnel in training". J.E.-E. acknowledges funding from the University of the Basque Country UPV/EHU, postdoctoral fellowship ESPPOC 16/65 "Call for recruitment and specialization of Doctor Researchers 2016". M.M.D.P. and L.B.-D., acknowledge funding support from University of the Basque Country UPV/EHU, UFI11/32, and from Gobierno Vasco under Grupos Consolidados with Grant No. IT998-16. F.B.-L. acknowledges funding support from the Ramon y Cajal Programme (Ministerio de Economia y Competitividad), Spain. F.B.-L. and L.B.-D. acknowledge funding support from the European Union's Seventh Framework Programme (FP7) for Research, Technological Development and Demonstration under Grant agreement No. 604241 as well as Gobierno Vasco, Dpto. Industria, Innovacion, Comercio y Turismo under ELKARTEK 2015 with Grant No. KK-2015/0000088. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/604241 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | cell analysis | es_ES |
dc.subject | high-throughput | es_ES |
dc.subject | microfluidics | es_ES |
dc.subject | microtechnology | es_ES |
dc.subject | embryonic stem-cells | es_ES |
dc.subject | droplet-based microfluidics | es_ES |
dc.subject | high-throughput analysis | es_ES |
dc.subject | extracellular-matrix | es_ES |
dc.subject | soluble factors | es_ES |
dc.subject | differentiation | es_ES |
dc.subject | culture | es_ES |
dc.subject | microarray | es_ES |
dc.subject | contact | es_ES |
dc.subject | biology | es_ES |
dc.title | Microtechnologies for Cell Microenvironment Control and Monitoring | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | http://www.mdpi.com/2072-666X/8/6/166 | es_ES |
dc.identifier.doi | 10.3390/mi8060166 | |
dc.departamentoes | Química analítica | es_ES |
dc.departamentoes | Zoología y biología celular animal | es_ES |
dc.departamentoeu | Kimika analitikoa | es_ES |
dc.departamentoeu | Zoologia eta animalia zelulen biologia | es_ES |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).